Cinnamomum camphora seed oil was extracted via ultrasound assisted aqueous enzymatic extraction (UAAEE). Neutral protease was selected for the enzymolysis pretreatment. A Plackett–Burman design and Box–Behnken design were employed to obtain the optimal UAAEE conditions and predict maximum oil yield. The optimal UAAEE conditions, with an actual yield of 80.12 ± 1.35%, were as follows: 4 h incubation time, 400 rpm agitation rate, 10 mL/g water to solid ratio, 1.9% enzyme amount, 1.4 h ultrasound time, and 220 W ultrasound power. Analysis of fatty acid compositions demonstrated that C. camphora seed oil possessed high medium-chain triglyceride content. Analysis of physicochemical properties demonstrated that the oil extracted via UAAEE possessed a lower acid value in comparison with oil extracted using the Soxhlet extraction; furthermore, other indices showed no significant difference between the two methods. The nutritional properties analysis revealed that the oil obtained using UAAEE had remarkable antiatherogenic and hypocholesterolemic activities. These results clarified that UAAEE is an efficient and eco-benign method for extracting plant seed oil and also indicated that C. camphora seed oil is a medium-chain triglyceride oil, which can be used as an ingredient in milk formulae for infants or in other health-related food industry products.

通过超声辅助水酶法提取（UAAEE）提取樟脑种子油。选择中性蛋白酶进行酶解预处理。Plackett-Burman 设计和 Box-Behnken 设计用于获得最佳 UAAEE 条件并预测最大石油产量。最佳UAAEE条件，实际产率为80.12±1.35%，如下：孵育时间4 h，搅拌速度400 rpm，水固比10 mL/g，酶量1.9%，超声时间1.4 h，220 W 超声功率。脂肪酸组成分析表明C. camphora种子油具有较高的中链甘油三酯含量。理化性质分析表明，UAAEE提取的油酸值低于索氏提取油；此外，其他指标显示两种方法之间没有显着差异。营养特性分析表明，使用 UAAEE 获得的油具有显着的抗动脉粥样硬化和降胆固醇活性。这些结果阐明了UAAEE是一种高效、环保的植物种子油提取方法，还表明樟树种子油是一种中链甘油三酯油，可作为婴儿配方奶粉或其他食品中的成分。与健康相关的食品工业产品。