Deoxynivalenol (DON) is a mycotoxin mainly produced by Fusarium graminearum and Fusarium culmorum and is commonly found in cereals such as wheat, barley, oats, and their derivatives. Scientists have been working on different strategies for DON detoxification, with biological detoxification being an approach with growing interest. When evaluating the use of microorganisms for mycotoxin detoxification, different phenomena can occur, namely, biotransformation (by microorganism metabolism and by interaction with extracellular cell proteins) and adsorption on cell walls, both of which can be present. In this study, a fast, simple, reliable, and inexpensive method for total DON quantification (dissolved and adsorbed) in bacterial culture assays is presented. This method can be used in screenings designed for searching DON-biodegrading microorganisms without requiring the analysis of the metabolites produced. This method has a good recovery (80.2%), reproducibility (3.2%) and low limit of quantification (0.60 μg/ml) that allows quantification under a wide range of DON concentrations in microbiological culture assays.

脱氧雪腐镰刀菌烯醇 (DON) 是一种霉菌毒素，主要由禾谷镰刀菌和镰刀菌产生。并且通常存在于谷物中，例如小麦、大麦、燕麦及其衍生物。科学家们一直在研究不同的 DON 解毒策略，其中生物解毒是一种越来越受到关注的方法。在评估使用微生物进行霉菌毒素解毒时，可能会出现不同的现象，即生物转化（通过微生物代谢和与细胞外细胞蛋白的相互作用）和在细胞壁上的吸附，这两者都可能存在。在这项研究中，提出了一种快速、简单、可靠且廉价的方法，用于细菌培养测定中的总 DON 定量（溶解和吸附）。该方法可用于为搜索 DON 生物降解微生物而设计的筛选，而无需分析产生的代谢物。该方法具有良好的回收率（80.2%），