Drosophila E74 is an early gene located in the polytene chromosome 74EF puff position. E74 controls the production of late genes, indicating that it plays a crucial role in this cascade model. Nilaparvata lugens E74 is closely related to Diaphorina citri, Bemisia tabaci, and Laodelphax striatellus. After downregulating E74, molting, and nymphal mortality were increased, and ovarian development was delayed. Moreover, the expression of Vg was reduced at the transcriptional level, as measured by qRT-PCR, and the content of Vg protein was reduced, as detected by Western blotting. After downregulating E74, the expression of hormone-related genes, including Tai, βFtz-F1, Met, Kr-h1, UspA, UspB, E93, and Br, was changed. The expression of E74 was significantly decreased after downregulating hormone-related genes. When the expression of E74 and βFtz-F1 was downregulated together, nymph mortality and molting mortality were higher than those when E74 or βFtz-F1 was downregulated alone. Thus, E74 probably interacts with βFtz-F1 at the genetic level. In summary, this study showed that E74 plays a crucial role in the development, metamorphosis and reproduction of N. lugens, possibly via the interaction with βFtz-F1 at the genetic level. This study provides a basis for the development of new target-based pesticides and new methods for the effective control of N. lugens.

中文翻译：

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Nilaparvata lugens (Hemiptera: Delphacidae) E74的功能及其与βFtz-F1的相互作用

果蝇 E74 是位于多线染色体 74EF 泡位置的早期基因。E74 控制晚期基因的产生，表明它在这个级联模型中起着至关重要的作用。Nilaparvata lugens E74 与柑橘木虱、烟粉虱和灰飞虱密切相关。下调 E74 后，蜕皮和若虫死亡率增加，卵巢发育延迟。此外，通过 qRT-PCR 测量，Vg 的表达在转录水平降低，而通过蛋白质印迹检测，Vg 蛋白的含量降低。下调E74后，激素相关基因Tai、βFtz-F1、Met、Kr-h1、UspA、UspB、E93和Br的表达发生了变化。下调激素相关基因后E74的表达显着降低。当E74和βFtz-F1同时下调时，若虫死亡率和蜕皮死亡率高于E74或βFtz-F1单独下调时的若虫死亡率和蜕皮死亡率。因此，E74 可能在遗传水平上与 βFtz-F1 相互作用。总之，本研究表明E74在褐飞虱的发育、变态和繁殖中发挥着至关重要的作用，可能是通过与βFtz-F1在遗传水平上的相互作用。该研究为开发新的靶向农药和有效控制褐飞虱的新方法提供了依据。褐飞虱的变态和繁殖，可能是通过与 βFtz-F1 在遗传水平上的相互作用。该研究为开发新的靶向农药和有效控制褐飞虱的新方法提供了依据。褐飞虱的变态和繁殖，可能是通过与 βFtz-F1 在遗传水平上的相互作用。该研究为开发新的靶向农药和有效控制褐飞虱的新方法提供了依据。