High-throughput probing macrophage–bacteria interactions at the single cell level with microdroplets,Lab on a Chip

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High-throughput probing macrophage–bacteria interactions at the single cell level with microdroplets
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-06-23 , DOI: 10.1039/d2lc00516f
Zhongyun Jiang ₁ , Sidi Liu ₁ , Xiang Xiao ₁ , Guimei Jiang ₁ , Qing Qu ₁ , Xingxing Miao ₁ , Renfei Wu ₁ , Rui Shi ₁ , Ruochen Guo ₁ , Jian Liu ₁

Affiliation

Pathogenic infections may lead to disruption of homeostasis, thus becoming a serious threat to the human health. Understanding the interactions between bacteria and macrophages is critical for therapeutic development against sepsis or inflammatory bowel disease. Here, we report a technique using droplet biosensors for the detection of nitric oxide (NO) secreted by a single macrophage under inflammatory stimuli. We demonstrated that the limit of detection can be promoted more than two orders of magnitude by our approach, in comparison to the conventional microplate format. The experiments of co-encapsulating single macrophages and different numbers of Escherichia coli (E. coli) enabled fluorescence monitoring of NO secretion by single macrophages over the incubation, and investigation of their interactions inside the isolated droplet for their separate fates. Our approach provides a unique platform to study the bacteria–macrophage interactions at the single cell level.

中文翻译：

用微滴在单细胞水平上高通量探测巨噬细胞-细菌相互作用

致病性感染可能导致体内平衡的破坏，从而成为对人类健康的严重威胁。了解细菌和巨噬细胞之间的相互作用对于败血症或炎症性肠病的治疗发展至关重要。在这里，我们报告了一种使用液滴生物传感器检测炎症刺激下单个巨噬细胞分泌的一氧化氮 (NO) 的技术。我们证明，与传统的微孔板格式相比，我们的方法可以将检测限提高两个数量级以上。共包封单个巨噬细胞和不同数量大肠杆菌（E.coli ）的实验) 能够荧光监测单个巨噬细胞在孵育过程中的 NO 分泌，并研究它们在分离液滴内的相互作用以了解它们各自的命运。我们的方法提供了一个独特的平台来研究单细胞水平的细菌-巨噬细胞相互作用。

更新日期：2022-06-23

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