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Endophytic bacteria from Euphorbia antiquorum L. protect Solanum lycopersicum L. against bacterial wilt caused by Ralstonia solanacearum
Egyptian Journal of Biological Pest Control ( IF 2.1 ) Pub Date : 2022-06-22 , DOI: 10.1186/s41938-022-00575-x
Diane Yimta Youmbi , Pierre Eke , Lanvin Rochal Kepngop Kouokap , Vanessa Nya Dinango , Gilbert Ghomsi Tamghe , Louise Nana Wakam , Fabrice Fekam Boyom

Bacterial wilt caused by Ralstonia solanacearum (syn. Pseudomonas solanacearum) is the most devastating bacterial disease of tomato (Solanum lycopersicum L.) occurring in tropical zones with consequential substantial yield losses in production fields. Currently, microbial technology in cropping systems has directed investigations toward biological control agents (BCAs), emphasizing the use of plant endophytes, including bacteria. The present work aimed to evaluate the potential of endophytic bacteria from Euphorbia antiquorum L. to suppress bacterial wilt incited by R. solanacearum in plants. Of the 10 endophytic species screened in vitro for their antagonistic activity, 6 exhibited promising potency with minimum inhibitory concentration (MIC) values ranging from 62.50 to 7.81 µg/ml. These species also produced cell wall-degrading enzymes (amylase, protease and cellulase), ammonia, siderophores, indole acetic acid and salicylic acid. The 3 most potent species (B. amyloliquefaciens CBa_RA37, B. velezensis CBv_BE1 and B. amyloliquefaciens CBa_BFL2) enhanced the germination of tomato seeds and protected young tomato plantlets from the devastating effects caused by R. solanacearum infection. This investigation demonstrated that these endophytic bacteria from E. antiquorum L. endowed with the ability to improve the growth and protection of tomato plants could be further developed as potential biopesticides to help mitigating the effect of bacterial wilt in tomato farms. Further investigation will set the baseline for formulation and evaluation of their efficacy in the open field.

中文翻译:

Euphorbia antiquorum L. 的内生细菌保护 Solanum lycopersicum L. 免受 Ralstonia solanacearum 引起的青枯病

由青枯病菌(同义词 Pseudomonas solanacearum)引起的青枯病是热带地区发生的最具破坏性的番茄(Solanum lycopersicum L.)细菌病害,导致生产领域的产量大幅下降。目前,种植系统中的微生物技术已将研究导向生物控制剂 (BCA),强调使用植物内生菌,包括细菌。目前的工作旨在评估大戟内生细菌抑制植物中青枯菌引起的青枯病的潜力。在体外筛选的 10 种内生菌的拮抗活性中,有 6 种表现出有希望的效力,最低抑菌浓度 (MIC) 值范围为 62.50 至 7.81 µg/ml。这些物种还产生细胞壁降解酶(淀粉酶、蛋白酶和纤维素酶)、氨、铁载体、吲哚乙酸和水杨酸。3 个最有效的物种(解淀粉芽孢杆菌 CBa_RA37、B.velezensis CBv_BE1 和解淀粉芽孢杆菌 CBa_BFL2)增强了番茄种子的萌发,并保护番茄幼苗免受青枯菌感染造成的破坏性影响。这项调查表明,这些来自 E. antiquorum L. 的内生细菌具有改善番茄植物生长和保护的能力,可以进一步开发为潜在的生物农药,以帮助减轻番茄农场青枯病的影响。进一步的调查将为制定和评估其在开放领域的功效设定基线。amyloliquefaciens CBa_RA37、B. velezensis CBv_BE1 和 B. amyloliquefaciens CBa_BFL2) 促进番茄种子的萌发并保护番茄幼苗免受青枯菌感染造成的破坏性影响。这项调查表明,这些来自 E. antiquorum L. 的内生细菌具有改善番茄植物生长和保护的能力,可以进一步开发为潜在的生物农药,以帮助减轻番茄农场青枯病的影响。进一步的调查将为制定和评估其在开放领域的功效设定基线。amyloliquefaciens CBa_RA37、B. velezensis CBv_BE1 和 B. amyloliquefaciens CBa_BFL2) 促进番茄种子的萌发并保护番茄幼苗免受青枯菌感染造成的破坏性影响。这项调查表明,这些来自 E. antiquorum L. 的内生细菌具有改善番茄植物生长和保护的能力,可以进一步开发为潜在的生物农药,以帮助减轻番茄农场青枯病的影响。进一步的调查将为制定和评估其在开放领域的功效设定基线。这项调查表明,这些来自 E. antiquorum L. 的内生细菌具有改善番茄植物生长和保护的能力,可以进一步开发为潜在的生物农药,以帮助减轻番茄农场青枯病的影响。进一步的调查将为制定和评估其在开放领域的功效设定基线。这项调查表明,这些来自 E. antiquorum L. 的内生细菌具有改善番茄植物生长和保护的能力,可以进一步开发为潜在的生物农药,以帮助减轻番茄农场青枯病的影响。进一步的调查将为制定和评估其在开放领域的功效设定基线。
更新日期:2022-06-23
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