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Natural Tissue-Imprinted Biointerface for the Topographical Education of a Biomimetic Cell Sheet
Langmuir ( IF 3.7 ) Pub Date : 2022-06-22 , DOI: 10.1021/acs.langmuir.2c00439 Mengting Yao , Wei Xu 1 , Yilei Meng , Shuangshuang Chen 2 , Qinghua Lu 1
Langmuir ( IF 3.7 ) Pub Date : 2022-06-22 , DOI: 10.1021/acs.langmuir.2c00439 Mengting Yao , Wei Xu 1 , Yilei Meng , Shuangshuang Chen 2 , Qinghua Lu 1
Affiliation
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Cell sheet engineering as a cell-based scaffold-free therapy is promising in tissue engineering, allowing precise transforming treatments for various tissue damage. However, the current cutting-edge techniques are still hampered by the difficulty in mimicking the natural tissue organizations and the corresponding physiological functions. In this work, cell-imprinting technology using the natural tissue as a template was proposed to rationally educate the cellular alignment in the cell sheet. Through this technique, we obtained temporary templates with morphological structure complementary to native tissues and then directly transferred the structure on the template to the collagen layer on a photothermally convertible substrate by secondary imprinting replication. The resultant biomimetic interface was used for cell culture and release to obtain a cell sheet with a texture similar to the natural tissue morphology. Different from conventional photolithography, the natural tissue-imprinted biointerface guides the geometry of cell sheets in the way of natural principles instead of stereotyped or overuniform cell organization. Simultaneously, a near-infrared laser (NIR) was used to irradiate the photothermally responsive substrate to obtain complete cell sheets efficiently and nondestructively. The natural tissue-educated myocardium cell sheets exhibited good physiological activity and biomimetic biofunctions, such as mechanical properties and physiological performances. This approach might open an inspiring prospect in regenerative medicine and offer a new approach to realizing the biomimetic tissue construction.
中文翻译：
用于仿生细胞片地形学教育的天然组织印迹生物界面
细胞片工程作为一种基于细胞的无支架疗法，在组织工程中很有前景，可以对各种组织损伤进行精确的转化治疗。然而，目前的尖端技术仍然受到难以模仿自然组织组织和相应的生理功能的阻碍。在这项工作中，提出了以天然组织为模板的细胞印迹技术，以合理地教育细胞片中的细胞排列。通过这种技术，我们获得了与天然组织互补的形态结构的临时模板，然后通过二次印迹复制将模板上的结构直接转移到光热转换基板上的胶原层上。所得仿生界面用于细胞培养和释放，以获得具有类似于天然组织形态的纹理的细胞片。与传统的光刻不同，自然组织印迹生物界面以自然原理的方式引导细胞片的几何形状，而不是刻板或过度均匀的细胞组织。同时，利用近红外激光（NIR）照射光热响应基板，高效、无损地获得完整的细胞片。天然组织培养的心肌细胞片表现出良好的生理活性和仿生生物功能，如机械性能和生理性能。
更新日期：2022-06-22
中文翻译：
用于仿生细胞片地形学教育的天然组织印迹生物界面
细胞片工程作为一种基于细胞的无支架疗法，在组织工程中很有前景，可以对各种组织损伤进行精确的转化治疗。然而，目前的尖端技术仍然受到难以模仿自然组织组织和相应的生理功能的阻碍。在这项工作中，提出了以天然组织为模板的细胞印迹技术，以合理地教育细胞片中的细胞排列。通过这种技术，我们获得了与天然组织互补的形态结构的临时模板，然后通过二次印迹复制将模板上的结构直接转移到光热转换基板上的胶原层上。所得仿生界面用于细胞培养和释放，以获得具有类似于天然组织形态的纹理的细胞片。与传统的光刻不同，自然组织印迹生物界面以自然原理的方式引导细胞片的几何形状，而不是刻板或过度均匀的细胞组织。同时，利用近红外激光（NIR）照射光热响应基板，高效、无损地获得完整的细胞片。天然组织培养的心肌细胞片表现出良好的生理活性和仿生生物功能，如机械性能和生理性能。
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