Membranes and filters made of nanofibers can have many medicines and water treatment applications. The use of silver nanoparticles (AgNPs) with antibacterial activity in these structures improve their efficiency. However, due to the toxicity of the compounds used in the chemical synthesis of AgNPs, in this study, AgNPs were obtained through a biological process using Fusarium sporotrichioides. AgNPs preparation conditions were optimized, including F. sporotrichioides medium and AgNO₃ concentration. Next, a PVA nanofiber membrane with bentonite and AgNPs (Bio-AgNPs or Chem-AgNPs) was prepared using electrospinning. The optimal conditions for the production of Bio-AgNPs were the culture of F. sporotrichioides in the MGYP culture medium and 12 M of AgNO₃. The Bio-AgNPs particle size and zeta potential were 58 nm and − 16.8 mV, respectively, with antibacterial activity. The PVA/NB/AgNPs nanofibers operation conditions included 7.5% w/w PVA, 3% w/w bentonite, and AgNPs 5% w/w at a voltage of 11 kV, feed rate of 0.5 mL/h, and 15 cm distance between the needle and the collector. The average diameter of the PVA/NB/Bio-AgNPs nanofibers was 230 nm. Also, the presence of silver in the nanofibers was confirmed through EDX and XRD methods. The antibacterial assay of the nanofibers showed that the inhibition zone of PVA/NB/Bio-AgNPs against E. coli and S. aureus was 0.62 and 0.36 mm, which is better than PVA/NB/Chem-AgNPs and comparable with chloramphenicol. The produced membrane is suitable for water treatment, food packaging, and wound dressing because of its good thermal, mechanical, and antibacterial properties.

由纳米纤维制成的膜和过滤器可以有许多药物和水处理应用。在这些结构中使用具有抗菌活性的银纳米粒子 (AgNPs) 可提高其效率。然而，由于用于化学合成 AgNPs 的化合物的毒性，在本研究中，AgNPs 是通过使用Fusarium sporotrichioides 的生物过程获得的。优化了 AgNPs 的制备条件，包括F. sporotrichioides培养基和AgNO ₃浓度。接下来，使用静电纺丝制备具有膨润土和 AgNPs（Bio-AgNPs 或 Chem-AgNPs）的 PVA 纳米纤维膜。生产 Bio-AgNPs 的最佳条件是F的培养。MGYP培养基和 12 M AgNO ₃中的 sporotrichioides 。Bio-AgNPs 粒径和 zeta 电位分别为 58 nm 和 - 16.8 mV，具有抗菌活性。PVA/NB/AgNPs 纳米纤维的操作条件包括 7.5% w/w PVA、3% w/w 膨润土和 AgNPs 5% w/w，电压为 11 kV，进料速率为 0.5 mL/h，距离为 15 cm在针和收集器之间。PVA/NB/Bio-AgNPs纳米纤维的平均直径为230 nm。此外，通过 EDX 和 XRD 方法证实了纳米纤维中银的存在。纳米纤维的抗菌试验表明 PVA/NB/Bio-AgNPs 对大肠杆菌的抑制区。大肠杆菌和金黄色葡萄球菌分别为 0.62 和 0.36 mm，优于 PVA/NB/Chem-AgNPs，与氯霉素相当。所生产的膜因其良好的热、机械和抗菌性能而适用于水处理、食品包装和伤口敷料。