Abstract

Unexplained recurrent pregnancy loss (RPL) composed almost half of all diagnosed miscarriage cases. As the apoptosis pathway is involved in the pregnancy process the present investigation aimed to assess the differential expression of the BCL-2 gene, SRA lncRNA, miR-361-3p in unexplained RPL patients. In this study, RNA was isolated from 50 blood samples of people with a history of RPL, and 50 blood samples of people with healthy fertility. After cDNA synthesis from these samples, alterations in the expression levels of the above-mentioned genes were examined by Real-Time PCR. Our results showed that the expression of BCL-2 and lncRNA SRA was significantly higher in the blood samples of RPL patients than in controls, while the expression of miR-361-3p was significantly downregulated. Besides, there were significant correlations between the changes in the expression of lncRNA SRA and miR-361-3p with BCL-2, in positive and negative directions, respectively. Also, miR-361-3p presented as a good diagnostic marker with the highest AUC value to discriminate between RPL and the healthy control subjects. These results proposed that ncRNAs may have a significant role in the regulation of apoptosis relates genes expression in RPL.

摘要

原因不明的复发性流产（RPL）几乎占所有确诊流产病例的一半。由于细胞凋亡途径参与妊娠过程，本研究旨在评估 BCL-2 基因、SRA lncRNA、miR-361-3p 在不明原因的 RPL 患者中的差异表达。在这项研究中，从 50 个有 RPL 病史的人的血液样本和 50 个具有健康生育能力的人的血液样本中分离出 RNA。从这些样品合成 cDNA 后，通过实时 PCR 检测上述基因表达水平的变化。我们的研究结果表明，RPL 患者血液样本中 BCL-2 和 lncRNA SRA 的表达显着高于对照组，而 miR-361-3p 的表达显着下调。除了，lncRNA SRA 和 miR-361-3p 的表达变化与 BCL-2 分别呈正向和负向显着相关。此外，miR-361-3p 作为具有最高 AUC 值的良好诊断标志物呈现以区分 RPL 和健康对照受试者。这些结果表明ncRNA可能在调节细胞凋亡相关基因表达中具有重要作用。