The airborne transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) via respiratory fluids and droplets suggests that mouthwashes containing substances with virucidal activity can help reduce viral spread. We conducted a multicenter, double-blind, placebo-controlled, randomized trial to assess the virucidal activity of cetylpyridinium chloride (CPC) mouthwashes. Outpatients who tested positive for SARS-CoV-2 infection with or without symptoms were randomized to perform washes and gargles for 1 min with 15 mL of either colored distilled water or 0.07% CPC (Vitis CPC Protect) mouthwash. The study outcomes were the SARS-CoV-2 log₁₀ viral RNA load and the nucleocapsid protein levels, both in saliva at 1 and 3 h after the intervention. In total, 118 patients were enrolled and randomized (mean [SD], age 46 [14] y). Thirteen of 118 participants (11%) did not complete follow-up or had insufficient sample volume for testing and were excluded from the analysis. The assessment of the viral load showed no significant differences between groups at any of the investigated points. However, the levels of SARS-CoV-2 nucleocapsid protein of lysed viruses were significantly higher in the CPC group compared with the control group at 1 h (adjusted difference 269.3 pg/mL; 95% confidence interval [CI], 97.1–441.5) and at 3 h postintervention (561.1 pg/mL; 95% CI, 380.0–742.2). In nonhospitalized patients with asymptomatic or mild symptomatic SARS-CoV-2 infection, a 0.07% CPC mouthwash, compared to placebo, was associated with a significant increase of nucleocapsid protein levels in saliva, indicating enhanced disruption of viral particles.

严重急性呼吸系统综合症冠状病毒 2 (SARS-CoV-2) 通过呼吸道液体和飞沫在空气中传播，这表明含有具有杀病毒活性物质的漱口水有助于减少病毒传播。我们进行了一项多中心、双盲、安慰剂对照、随机试验，以评估氯化十六烷基吡啶 (CPC) 漱口水的杀病毒活性。SARS-CoV-2 感染检测呈阳性且有或无症状的门诊患者随机接受 15 mL 有色蒸馏水或 0.07% CPC (Vitis CPC Protect) 漱口水清洗和漱口 1 分钟。研究结果是 SARS-CoV-2 log ₁₀干预后 1 和 3 小时唾液中的病毒 RNA 载量和核衣壳蛋白水平。总共有 118 名患者被纳入并随机分组（平均 [SD]，年龄 46 [14] 岁）。118 名参与者中有 13 名 (11%) 未完成随访或没有足够的样本量进行测试，因此被排除在分析之外。病毒载量的评估显示，在任何研究点，组间均无显着差异。然而，在 1 h 时，CPC 组裂解病毒的 SARS-CoV-2 核衣壳蛋白水平显着高于对照组（调整后差异 269.3 pg/mL；95% 置信区间 [CI]，97.1–441.5）和干预后 3 小时（561.1 pg/mL；95% CI，380.0–742.2）。在无症状或轻度症状 SARS-CoV-2 感染的非住院患者中，使用 0.07% CPC 漱口水，