In 2021, we observed symptoms of black mould disease on dates cv. Piarom, the most economically valuable export date cultivar in Hormozgan province, southern Iran. This study aimed to evaluate the disease incidence, identify the aetiological agent, and fulfill the pathogenicity on Piarom dates in this area. Aspergillus isolates were permanently obtained from the symptomatic dates on PDA, which were identified as Aspergillus niger based on morphological characteristics, as well as partial sequence data from the internal transcribed spacer region (ITS1-5.8S-ITS2) and Beta Tubulin (benA) gene. A phylogeny inferred using individual sequence data from ITS and benA sequences placed our isolates together with the other A. niger strains in the GenBank. The pathogenicity assays using symptom-free Piarom dates revealed the association of A. niger with the disease. Reisolation from the inoculated fruits yielded the isolates of A. niger, proving Koch's postulates. This is the first report of A. niger causing black mould disease on Piarom dates in Iran.

2021 年，我们在枣 cv 上观察到了黑霉病的症状。Piarom，伊朗南部霍尔木兹甘省最具经济价值的出口枣品种。本研究旨在评估该地区Piarom枣的发病率，确定病因，并实现致病性。从 PDA 上的症状日期中永久获得曲霉分离物，根据形态特征以及来自内部转录间隔区 (ITS1-5.8S-ITS2) 和 Beta Tubulin ( benA ) 基因的部分序列数据将其鉴定为黑曲霉. 使用来自 ITS 和benA序列的单个序列数据推断的系统发育将我们的分离物与其他A. niger放在一起GenBank 中的菌株。使用无症状 Piarom 枣的致病性测定揭示了A. niger与该疾病的关联。从接种的果实中重新分离产生了黑曲霉的分离物，证明了科赫的假设。这是伊朗第一次报道A. niger在 Piarom 枣上引起黑霉病。