To enhance the performance of moving bed biofilm reactor (MBBR) inoculated with heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria, bioaugmentation with Acinetobacter sp. TAC-1 was firstly employed and then the treatment performance for real swine wastewater was presented in this study. Results indicated that NH₄⁺-N and TN removal rates of bioaugmented reactor were significantly improved from 16.53 mg/L/h and 16.15 mg/L/h to 24.58 mg/L/h and 24.45 mg/L/h, respectively. The efficient removal performance (NH₄⁺-N 95.01%, TN 86.40%) for real swine wastewater was achieved within 24 h. Microbial analysis indicated that the composition of functional bacteria varied with the introduction of Acinetobacter sp. TAC-1, especially the abundance of Acinetobacter, Paracoccus and Rhodococcus related to the nitrogen removal. Furthermore, bioaugmentation with Acinetobacter sp. TAC-1 increased abundance of enzymes and functional genes (nirS, nirK and norZ) corresponding to denitrification that may be responsible for the enhanced nitrogen removal performance.

为了提高接种异养硝化-好氧反硝化 (HN-AD) 细菌的移动床生物膜反应器 (MBBR) 的性能，用不动杆菌进行生物强化。本研究首先采用 TAC-1，然后介绍了真实猪废水的处理性能。结果表明，生物强化反应器的NH ₄ ⁺ -N和TN去除率分别从16.53 mg/L/h和16.15 mg/L/h显着提高到24.58 mg/L/h和24.45 mg/L/h。在24小时内实现了对真实猪废水的有效去除性能（NH ₄ ⁺ -N 95.01%，TN 86.40%）。微生物分析表明，功能菌的组成随着不动杆菌的引入而变化sp。TAC-1，尤其是不动杆菌、副球菌和红球菌的丰度与脱氮有关。此外，用不动杆菌属进行生物强化。TAC-1 增加了与反硝化作用相对应的酶和功能基因（nirS、nirK 和 norZ）的丰度，这可能是提高脱氮性能的原因。