As one of the serious threats to food safety and public health, heavy metal contamination in food and agriculture-related matrices has aroused serious concern for the public and needs to be solved urgently. In this regard, it is important to focus on developing ingenious assays for facile, reliable, efficient, and accurate reporting the residual levels of heavy metals in food and agriculture-related matrices. Recently, with the prosperous progress of fluorescence assays, fluorescence resonance energy transfer (FRET)-based assays have been emerging as one of the most promising analytical tools to recognize and quantify the fouling of heavy metals in food and agriculture-related matrices, which exhibits a simple, rapid, cost-effective, reliable, stable, sensitive, selective, and real-time way. Regarding the vast endeavors that have been contributed in this field up to now, we here summarize the recent advances of FRET-based innovative assays for reporting the contamination of heavy metals (e.g., Hg, Cu, Pb, Fe, Ag, Cd, Cr, As, U, and their mixture) in complicated food and agriculture-related matrices, point out the unavoidable challenges, and propose the future perspectives. This review might give important insights for developing effective warning-early mechanisms, management strategies, and commercial FRET-based point-of-care assays toward heavy metal contamination in food and agriculture-related matrices, safeguarding food safety and public health.

作为食品安全和公众健康的严重威胁之一，食品和农业相关基质中的重金属污染已引起公众的高度关注，亟待解决。在这方面，重要的是要专注于开发巧妙的检测方法，以简便、可靠、高效和准确地报告食品和农业相关基质中的重金属残留水平。近年来，随着荧光分析的蓬勃发展，基于荧光共振能量转移 (FRET) 的分析已成为识别和量化食品和农业相关基质中重金属污染的最有前途的分析工具之一。一种简单、快速、经济、可靠、稳定、灵敏、选择性和实时的方法。例如，Hg、Cu、Pb、Fe、Ag、Cd、Cr、As、U 及其混合物）在复杂的粮食和农业相关基质中，指出不可避免的挑战，并提出未来展望。这篇综述可能会为制定有效的预警机制、管理策略和基于商业 FRET 的即时检测分析食品和农业相关基质中的重金属污染、保障食品安全和公众健康提供重要见解。