The antioxidant properties of Se-containing peptides from Cardamine enshiensis (SeCPPs) and their impact on gut microbiota were studied in d-galactose (d-gal)- injected mice and antibiotic-treated mice. The structures of SeCPPs were identified by UPLC-Q-Extractive Orbitrap MS. In d-gal ageing mice, SeCPPs were associated with significantly decreased acetyl cholinesterase (AchE) activity, malondialdehyde (MDA) content, increased glutathione peroxidase (GSH-Px) activity, downregulated tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-1β (IL-1β) levels (p < 0.05), and improved memory. In antibiotic-treated mice, SeCPPs were associated with reduced Proteobacteria and evaluated Akkermansia abundances (p < 0.01). Eighty-five Se-containing peptides were identified in SeCPPs. Peptides such as RV-SeM-I, RA-SeM-T and R-SeC-K showed low binding energy with 1,1-diphenyl-2-picrylhydrazyl (DPPH), and their binding affinities were confirmed by molecular docking. Overall, compared with Na₂SeO₃ and SeMet, SeCPPs showed superior antioxidant activity via their association with higher antioxidant enzyme activity, scavenging free radical properties and gut microbiome modulation.

在注射d-半乳糖 ( d -gal) 的小鼠和抗生素处理的小鼠中研究了来自Cardamine enshiensis (SeCPPs) 的含硒肽的抗氧化特性及其对肠道微生物群的影响。通过 UPLC-Q-Extractive Orbitrap MS 鉴定了 SeCPP 的结构。在d -gal 衰老小鼠中，SeCPP 与乙酰胆碱酯酶 (AchE) 活性、丙二醛 (MDA) 含量显着降低、谷胱甘肽过氧化物酶 (GSH-Px) 活性增加、肿瘤坏死因子-α (TNF-α) 下调、白细胞介素- 6 (IL-6) 和白细胞介素-1β (IL-1β) 水平 ( p  < 0.05)，并改善记忆力。在抗生素治疗的小鼠中，SeCPPs 与减少的变形菌有关并评估了 Akkermansia的丰度 ( p  < 0.01)。在 SeCPP 中鉴定出 85 种含硒肽。RV-SeM-I、RA-SeM-T 和 R- SeC -K 等肽与 1,1-二苯基-2-苦基肼 (DPPH ) 的结合能较低，分子对接证实了它们的结合亲和力。总体而言，与 Na _{2 SeO 3}和 SeMet 相比，SeCPPs通过与更高的抗氧化酶活性、清除自由基特性和肠道微生物组调节相关，显示出优异的抗氧化活性。