Abstract

This work analyses the adhesion of flagellated microalgae to seven surfaces that have different water adhesion tension characteristics. Chlamydomonas reinhardtii and Isochrysis galbana, were cultivated in batch and fed-batch mode at four nitrogen/phosphorus (N/P) ratios (from 1.29 to 70) and subjected to four irradiance levels (50, 100, 200 and 400 µE·s⁻¹·m⁻²) at 23 °C. Cell adhesion was greater in C. reinhardtii and a higher biomass concentration was obtained for this strain, reaching 2 g·L⁻¹ compared to 1 g·L⁻¹ for I. galbana. The adhesion of cells and exopolymeric substances was measured upon the batch and the first fed-batch reaching the stationary growth phase, observing a direct correlation between them and inversely to biomass generation in the cultures. The protein adhesion data for the different materials are comparable to those for cell adhesion coinciding with minimums of Baier's theory and Vogler. It is observed displacements in the curves as a function of the irradiance level.

摘要

这项工作分析了鞭毛微藻对具有不同水粘附张力特性的七个表面的粘附。Chlamydomonas reinhardtii和Isochrysis galbana在四种氮/磷 (N/P) 比率（从 1.29 到 70）下分批和补料分批培养，并经受四种辐照度水平（50、100、200 和 400 µE·s ^{- 1} ·m ^-2 ) 在 23 °C。C. reinhardtii中的细胞粘附更大，并且该菌株获得了更高的生物量浓度，达到 2 g·L ^-1与I. galbana的 1 g·L ^-1相比. 在批次和第一个补料批次达到稳定生长期时测量细胞和外聚合物的粘附，观察它们之间的直接相关性，与培养物中的生物质产生成反比。不同材料的蛋白质粘附数据与细胞粘附的数据相当，符合 Baier 理论和 Vogler 的最小值。可以观察到曲线中的位移是辐照度水平的函数。