Taking Phellodendron chinense Schneid (PcS) as the raw material with ultrasonic-assisted eutectic solvent, the effects of various DESs on the extractable content of palmatine and berberine in PcS were investigated. On the basis of the single-factor test, the best DES was determined to be choline chloride and 1,3-propanediol (mole ratio 1 : 2). After optimizing by the response surface method, the optimum extraction conditions were as follows: the solid-liquid ratio was 1 : 30 (w/v), water content was 30% (v/v), vortex time was 7 min, ultrasonic time was 20 min, ultrasonic temperature was 60°C, ultrasonic power was 400 W, and the content of palmatine in PcS was 5.421 ± 0.283 mg/g, and the content of berberine in PcS was 15.573 ± 0.539 mg/g. Therefore, DES prepared from choline chloride and 1,3-propanediol can be used to extract palmatine and berberine from PcS. The optimized process conditions determined by the response surface method are reliable and can provide a reference for the green extraction of effective components from PcS.

中文翻译：

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响应面法优化深共晶溶剂提取黄柏有效成分

服用黄柏以施耐德（PcS）为原料，采用超声辅助共熔溶剂，考察了各种DESs对PcS中巴马汀和黄连素可提取物含量的影响。在单因素试验的基础上，确定最佳DES为氯化胆碱和1,3-丙二醇（摩尔比1:2）。经响应面法优化后，最佳提取条件为：固液比为1：30（w/v），含水量为30%（v/v），涡旋时间为7 min，超声时间为20 min，超声温度60℃，超声功率400 W，PcS中巴马汀含量为5.421±0.283 mg/g，PcS中黄连素含量为15.573±0.539 mg/g。因此，由氯化胆碱和1,3-丙二醇制备的DES可用于从PcS中提取巴马汀和小檗碱。