TMT-Based Quantitative Proteomics Analyses Reveal the Antibacterial Mechanisms of Anthocyanins from Aronia melanocarpa against Escherichia coli O157:H7,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

TMT-Based Quantitative Proteomics Analyses Reveal the Antibacterial Mechanisms of Anthocyanins from Aronia melanocarpa against Escherichia coli O157:H7
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-21 , DOI: 10.1021/acs.jafc.2c02742
Haotian Deng ₁ , Bo Xue ₁ , Mingyue Wang ₁ , Yuqi Tong ₁ , Chang Tan ₁ , Meizhi Wan ₁ , Yanwen Kong ₁ , Xianjun Meng ₁ , Jinyan Zhu ₂

Affiliation

Aronia melanocarpa anthocyanins (AMAs), as natural plant extracts, can control pathogens and are attracting increasing attention. In this study, a tandem mass tag (TMT) quantitative proteomics method combined with multiple reaction monitoring (MRM) was used to explore the antibacterial mechanism of AMAs against Escherichia coli at the protein level. The results showed that 1739 proteins were identified in E. coli treated with AMAs, of which 628 were altered, including 262 downregulated proteins and 366 upregulated proteins. Bioinformatics analysis showed that these differentially expressed proteins have different molecular functions and participate in different molecular pathways. AMAs can affect E. coli protein biosynthesis, DNA replication and repair, oxidative stress response, peptidoglycan biosynthesis, and homeostasis. These pathways induce morphological changes and cell death. The results of this study help understand the molecular mechanism of the inhibitory effect of AMAs on food-borne pathogens and provide a reference for further development of plant-derived antimicrobial agents.

中文翻译：

基于 TMT 的定量蛋白质组学分析揭示了黑果花中花青素对大肠杆菌 O157:H7 的抗菌机制

Aronia melanocarpa花青素 (AMAs) 作为天然植物提取物，可以控制病原体并越来越受到关注。本研究采用串联质量标签（TMT）定量蛋白质组学方法结合多反应监测（MRM）从蛋白质水平探讨AMAs对大肠杆菌的抗菌机制。结果显示，经AMA处理的大肠杆菌中鉴定出1739个蛋白质，其中628个发生改变，包括262个下调蛋白质和366个上调蛋白质。生物信息学分析表明，这些差异表达的蛋白质具有不同的分子功能，参与不同的分子通路。AMA 会影响大肠杆菌蛋白质生物合成、DNA 复制和修复、氧化应激反应、肽聚糖生物合成和体内平衡。这些途径诱导形态变化和细胞死亡。本研究结果有助于了解AMAs对食源性病原体抑制作用的分子机制，为进一步开发植物源性抗菌药物提供参考。

更新日期：2022-06-21

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11