Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration,Lab on a Chip

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Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-06-21 , DOI: 10.1039/d2lc00213b
Alexander T Hwu ₁ , Masoud Madadelahi ₂ , Rie Nakajima ₃ , Ehsan Shamloo ₄ , Alexandra Perebikovsky ₄ , Horacio Kido ₄ , Aarti Jain ₃ , Algis Jasinskas ₃ , Shawna Prange ₅ , Philip Felgner ₃ , Marc Madou _{1,

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Affiliation

Heterogeneous immunoassays (HI) are an invaluable tool for biomarker detection and remain an ideal candidate for microfluidic point-of-care diagnostics. However, automating and controlling sustained fluid flow from benchtop to microfluidics for the HI reaction during the extended sample incubation step, remains difficult to implement; this leads to challenges for assay integration and assay result interpretation. To address these issues, we investigated the liquid reciprocation process on a microfluidic centrifugal disc (CD) to generate continuous, bidirectional fluid flow using only a rotating motor. Large volumetric flow rates (μL s⁻¹) through the HI reaction chamber were sustained for extended durations (up to 1 h). The CD liquid reciprocation operating behavior was characterized experimentally and simulated to determine fluid flow shear rates through our HI reaction chamber. We demonstrated the continuous CD liquid reciprocation for target molecule incubation for a microarray HI and that higher fluid shear rates negatively influenced our fluorescence intensity. We highlight the importance of proper fluid flow considerations when integrating HIs with microfluidics.

中文翻译：

微流控集成过程中抗体与 COVID 抗原阵列结合的离心盘液体往复流动考虑

异质免疫分析 (HI) 是生物标志物检测的宝贵工具，并且仍然是微流体即时诊断的理想候选者。然而，在延长的样品孵育步骤中，自动化和控制从台式到微流体的持续流体流动以进行 HI 反应，仍然难以实施。这给分析集成和分析结果解释带来了挑战。为了解决这些问题，我们研究了微流体离心盘 (CD) 上的液体往复运动过程，以仅使用旋转电机产生连续的双向流体流动。大体积流量（μL s ^-1) 通过 HI 反应室持续较长时间（最多 1 小时）。CD液体往复操作行为通过实验和模拟来确定通过我们的HI反应室的流体流动剪切速率。我们展示了用于微阵列 HI 的目标分子孵育的连续 CD 液体往复运动，并且更高的流体剪切速率对我们的荧光强度产生了负面影响。在将 HI 与微流体集成时，我们强调了适当的流体流动考虑的重要性。

更新日期：2022-06-23

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