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Portable and sensitive detection of cancer cells via a handheld luminometer
Analyst ( IF 3.6 ) Pub Date : 2022-06-21 , DOI: 10.1039/d2an00666a Jing Lei 1 , Lu Shi 1 , Wei Liu 1 , Baoxin Li 1 , Yan Jin 1
Analyst ( IF 3.6 ) Pub Date : 2022-06-21 , DOI: 10.1039/d2an00666a Jing Lei 1 , Lu Shi 1 , Wei Liu 1 , Baoxin Li 1 , Yan Jin 1
Affiliation
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Portable detection of cancer cells is important for early-stage diagnostic applications and prognosis of cancer. Herein, a simple and sensitive chemiluminescence method was proposed for portable detection of cancer cells via a handheld luminometer. It is based on the cancer cell triggered cyclic strand displacement reaction (SDR) for signal amplification. Cancer cells, CCRF-CEM, bind to their aptamer and release the trigger DNA (TDNA) of SDR. The TDNA initiates the cyclic SDR between magnetic bead modified hairpin DNA 1 (MB-H1) and HRP-tagged hairpin DNA 2 (HRP-H2), resulting in an enrichment of HRP onto the surface of magnetic beads by forming H1/H2 duplexes. HRP catalyzed luminol/H2O2/PIP solution to produce a strong chemiluminescence (CL) signal. CCRF-CEM cells were sensitively detected by combining magnetic enrichment with the signal amplification of SDR. The CL intensity showed an excellent linear relationship with the number of CCRF-CEM cells in the range of 100 to 5 × 104 cells per mL. The detection limit was as low as 85 cells per mL. Therefore, it offers a sensitive, cheap and portable method for the chemiluminescence detection of cancer cells and provides a new option for the early diagnosis of cancers.
中文翻译：
通过手持式光度计进行便携式和灵敏的癌细胞检测
癌细胞的便携式检测对于癌症的早期诊断应用和预后非常重要。在此，提出了一种简单而灵敏的化学发光方法，用于通过手持式光度计便携式检测癌细胞。它基于癌细胞触发的环链置换反应（SDR）进行信号放大。癌细胞 CCRF-CEM 与其适体结合并释放 SDR 的触发 DNA (TDNA)。TDNA 启动磁珠修饰的发夹 DNA 1 (MB-H1) 和 HRP 标记的发夹 DNA 2 (HRP-H2) 之间的循环 SDR，通过形成 H1/H2 双链体将 HRP 富集到磁珠表面。HRP催化鲁米诺/H 2 O 2/PIP 溶液可产生强化学发光 (CL) 信号。结合磁富集和SDR的信号放大，对CCRF-CEM细胞进行灵敏检测。CL 强度与CCRF-CEM 细胞数在每毫升100 至5 × 10 4 个细胞的范围内表现出极好的线性关系。检测限低至每毫升 85 个细胞。因此，它为癌细胞的化学发光检测提供了一种灵敏、廉价、便携的方法，为癌症的早期诊断提供了新的选择。
更新日期：2022-06-21
中文翻译：
通过手持式光度计进行便携式和灵敏的癌细胞检测
癌细胞的便携式检测对于癌症的早期诊断应用和预后非常重要。在此，提出了一种简单而灵敏的化学发光方法，用于通过手持式光度计便携式检测癌细胞。它基于癌细胞触发的环链置换反应（SDR）进行信号放大。癌细胞 CCRF-CEM 与其适体结合并释放 SDR 的触发 DNA (TDNA)。TDNA 启动磁珠修饰的发夹 DNA 1 (MB-H1) 和 HRP 标记的发夹 DNA 2 (HRP-H2) 之间的循环 SDR，通过形成 H1/H2 双链体将 HRP 富集到磁珠表面。HRP催化鲁米诺/H 2 O 2/PIP 溶液可产生强化学发光 (CL) 信号。结合磁富集和SDR的信号放大，对CCRF-CEM细胞进行灵敏检测。CL 强度与CCRF-CEM 细胞数在每毫升100 至5 × 10 4 个细胞的范围内表现出极好的线性关系。检测限低至每毫升 85 个细胞。因此，它为癌细胞的化学发光检测提供了一种灵敏、廉价、便携的方法，为癌症的早期诊断提供了新的选择。
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