The objectives of the present study were to prepare an active biodegradable film based on zein-containing copper oxide nanoparticles (CuO NPs) and Mentha longifolia var. asiatica essential oil (MAEO) and to investigate the physicochemical and structural properties of the produced films. Zein films were prepared by solvent evaporation method and adding CuO NPs and MAEO in different concentrations of 0, 2.5, and 5% (w/w) and 0, 1.5, and 3% (w/w), respectively. The properties of the prepared nanocomposite including thickness, water vapor permeability, solubility, moisture absorption, moisture content, antioxidant properties, color indices and mechanical and antimicrobial properties were investigated. TGA, FT-IR, and FESEM tests were performed to investigate the interaction of film compositions, thermal stability and film surface morphology, respectively. The results showed that increasing the amount of CuO NPs led to a cohesive and smooth surface in the film, which improved the water vapor permeability in the films compared to the control film. As the percentage of CuO NPs and MAEO increased, the solubility and the moisture content decreased, but the thickness and antioxidant content of the film increased compared to the control sample (from 60.1 to 86.9%). Also, increasing the percentage of CuO NPs and MAEO led to the improvement of the mechanical properties of the film. By increasing the concentration of MAEO and CuO NPs, the amount of color indices (b* and a*) decreased and then increased. The results of antimicrobial activity against S. aureus and E. coli showed that with the addition of CuO NPs and MAEO, the diameter of the growth inhibition zone increased and the films showed high antimicrobial activity.

本研究的目的是制备一种基于含有玉米醇溶蛋白的氧化铜纳米粒子 (CuO NPs) 和Mentha longifolia var 的活性可生物降解薄膜。亚细亚精油（MAEO），并研究所生产薄膜的物理化学和结构特性。通过溶剂蒸发法制备玉米醇溶蛋白薄膜，分别添加 0、2.5 和 5% (w/w) 和 0、1.5 和 3% (w/w) 不同浓度的 CuO NPs 和 MAEO。研究了制备的纳米复合材料的性能，包括厚度、水蒸气渗透性、溶解度、吸湿性、水分含量、抗氧化性能、颜色指数以及机械和抗菌性能。进行 TGA、FT-IR 和 FESEM 测试以分别研究薄膜成分、热稳定性和薄膜表面形态的相互作用。结果表明，增加CuO NPs的量会导致薄膜中的内聚和光滑表面，与对照膜相比，这提高了膜中的水蒸汽渗透性。随着 CuO NPs 和 MAEO 百分比的增加，溶解度和水分含量降低，但与对照样品相比，薄膜的厚度和抗氧化剂含量增加（从 60.1% 到 86.9%）。此外，增加 CuO NPs 和 MAEO 的百分比可以改善薄膜的机械性能。通过增加 MAEO 和 CuO NPs 的浓度，颜色指数的量 (b*和a* ) 减少然后增加。对金黄色葡萄球菌和大肠杆菌的抗菌活性结果表明，随着CuO NPs和MAEO的加入，生长抑制区的直径增加，薄膜表现出较高的抗菌活性。