Due to unique reaction conditions of the acoustic cavitation process, ultrasound-assisted synthesis of nanoparticles has attracted increased research attention. In this study, we demonstrate the effect of ultrasonic irradiation on the crystallinity, stability, biocompatibility, and magnetic properties of chitosan-coated superparamagnetic iron oxide nanoparticles (CS-SPIONs). CS solution and colloidal suspension of SPIONs were mixed and sonicated using an ultrasonic probe of 1.3 cm tip size horn, frequency (20 kHz), and power (750 W). Different samples were sonicated for 1.5, 5, and 10 min with corresponding acoustic powers of 67, 40 and 36 W, and the samples were denoted S_1.5, S_5, and S₁₀, respectively. The samples were characterized using X-ray diffractometer (XRD), Energy dispersive X-ray (EDX), Transmission electronic microscope (TEM), Fourier transform infrared spectroscopy (FTIR), Zeta sizer, and vibrating sample magnetometer (VSM). Cell cytotoxicity and cell uptake were investigated with human embryonic kidney 293 (HEK-293) cells through MTT assay and Prussian blue staining, respectively. The sharp peaks of the XRD pattern were disappearing with an increase in the sonication period but a decrease in acoustic power. EDX analysis also demonstrates that atomic and weight percentages of the various elements in the samples were decreasing with an increase in the sonication period. However, the Zeta potential (ζ) values increase with an increase in the sonication period.The saturation magnetization (M_s) of the S_1.5 before and after the coating is 62.95 and 86.93 emu/g, respectively. Cell cytotoxicity and uptake of the S_1.5 show that above 70% of cells were viable at the highest concentration and the longest incubation duration. Importantly, the CS-SPIONs synthesized by the sonochemical method are non-toxic and biocompatible.

由于声空化过程的独特反应条件，超声辅助合成纳米粒子引起了越来越多的研究关注。在这项研究中，我们展示了超声波辐照对壳聚糖包覆的超顺磁性氧化铁纳米粒子 (CS-SPIONs) 的结晶度、稳定性、生物相容性和磁性的影响。CS 溶液和 SPION 的胶体悬浮液混合并使用 1.3 cm 尖端尺寸喇叭、频率 (20 kHz) 和功率 (750 W) 的超声波探头进行超声处理。对不同的样品进行 1.5、5 和 10 分钟的超声处理，相应的声功率分别为 67、40 和 36 W，样品分别表示为 S _1.5、 S ₅和 S ₁₀， 分别。使用 X 射线衍射仪 (XRD)、能量色散 X 射线 (EDX)、透射电子显微镜 (TEM)、傅里叶变换红外光谱仪 (FTIR)、Zeta sizer 和振动样品磁力计 (VSM) 对样品进行表征。分别通过 MTT 测定和普鲁士蓝染色，用人胚肾 293 (HEK-293) 细胞研究细胞毒性和细胞摄取。XRD 图案的尖峰随着超声处理时间的增加而消失，但声功率降低。EDX 分析还表明，样品中各种元素的原子百分比和重量百分比随着超声处理时间的增加而降低。然而，Zeta 电位 (ζ) 值随着超声处理周期的增加而增加。饱和磁化强度 ( M_s ) 的 S _1​​.5在涂层前后分别为 62.95 和 86.93 emu/g。S _1.5的细胞细胞毒性和摄取表明，70% 以上的细胞在最高浓度和最长孵育时间下存活。重要的是，通过声化学方法合成的 CS-SPIONs 无毒且具有生物相容性。