In this study, a novel temperature responsive surfactant-free microemulsion (SFME) solvent was prepared and fully characterized. Combined with ultrasonic assisted extraction (UAE), it was applied to the simultaneous extraction and enrichment of different hydrophilic and hydrophobic components in Rosa roxburghii leaves. The extraction factors such as solid-liquid ratio, extraction temperature and extraction time were optimized by response surface methodology. The extraction yields of components with different polarity were improved by 1.03–3.90 folds compared with traditional solvents. The extraction mass transfer process was fitted by first-order kinetic model and second-order kinetic model. The results showed that the fitting effect of second-order kinetic model was better. Moreover, molecular dynamics revealed that hydrogen bonds were formed between the solvent and the compound molecules then promoted the improvement of extraction capacity. Notably, in situ separation and recovery of compounds with different polarities were conducted by SFME and the recovered extracts had good antioxidant capacity. In conclusion, this novel SFME was environment-friendly and simultaneously appropriate for the effective extraction and enrichment of natural active compounds from plant matrix.

在本研究中，制备并充分表征了一种新型温度响应型无表面活性剂微乳液 (SFME) 溶剂。结合超声辅助提取（UAE），应用于刺梨叶片中不同亲水和疏水成分的同时提取富集。采用响应面法优化料液比、提取温度和提取时间等提取因素. 与传统溶剂相比，不同极性组分的提取率提高了1.03-3.90倍。萃取传质过程采用一级动力学模型和二级动力学模型拟合。结果表明，二阶动力学模型的拟合效果较好。此外，分子动力学表明，溶剂和化合物分子之间形成氢键，从而促进了萃取能力的提高。值得注意的是，SFME对不同极性的化合物进行原位分离和回收，回收的提取物具有良好的抗氧化能力。总之，这种新型SFME对环境友好，同时适用于从植物基质中有效提取和富集天然活性化合物。