Fluorescence lifetime imaging microscopy (FLIM) is only related to the molecular structure and energy level distribution of the probe, not to the fluorescence intensity. It is an efficient imaging method, because it is not susceptible to interference from the internal environment of biological samples. Diabetes, as a systemic metabolic disease, causes various degrees of inflammation in organs and tissues. As we all know, inflammation of organ and tissue will affect cellular viscosity increases. In this work, a new amphiphilic molecular probe YF-V with a stable structure, good selectivity, fluorescence lifetime response and low cytotoxicity was designed. Under the condition of high viscosity, the rotation of the rotor and the twisting intramolecular charge transfer (TICT) mechanism were inhibited, leading to the extension of the fluorescence lifetime. In the cellular level, YF-V could sensitively detect the dynamic viscosity changes of cells induced by glucose through FLIM. Meanwhile, YF-V is also successfully applied to observe the difference in viscosity between the tissues and organs of diabetic mice and normal mice, and take lead in the detection of organ damage in diabetic mice with different disease durations. This provides an efficient and intuitive method for evaluating organ damage and early diagnosis in diabetes.

荧光寿命成像显微镜（FLIM）仅与探针的分子结构和能级分布有关，与荧光强度无关。它是一种高效的成像方法，因为它不易受到生物样品内部环境的干扰。糖尿病作为一种全身性代谢性疾病，会导致器官和组织出现不同程度的炎症。众所周知，器官和组织的炎症会影响细胞粘度的增加。在这项工作中，一种新的两亲分子探针YF-V设计了结构稳定、选择性好、荧光寿命响应好、细胞毒性低的材料。在高粘度条件下，转子的旋转和扭曲的分子内电荷转移（TICT）机制受到抑制，从而延长了荧光寿命。在细胞水平上，YF-V可以通过FLIM灵敏地检测葡萄糖诱导的细胞动态粘度变化。同时，YF-V也成功应用于观察糖尿病小鼠与正常小鼠组织器官黏度差异，率先检测不同病程糖尿病小鼠的器官损伤情况。这为评估糖尿病的器官损伤和早期诊断提供了一种有效且直观的方法。