Curcumin (CUR) was encapsulated into yeast cells (YCs) through a pH-driven method with a 5.04-fold increase in loading capacity and a 43.63-fold reduction in incubation time compared to the conventional diffusion method. Optimal encapsulation was obtained when the mass ratio of CUR to YCs was 0.1, and the loading capacity and encapsulation efficiency were 8.07% and 80.66%, respectively. Encapsulation of CUR into YCs was confirmed by fluorescence microscopy, differential scanning calorimetry, and thermogravimetric analysis. Fourier transform infrared spectroscopy and X-ray diffraction further demonstrated that the encapsulated CUR was interacted with mannoprotein and β-glucan of the cell wall network through hydrophobic interaction and hydrogen bond in amorphous state. The in vitro bioaccessibility of YCs-loaded CUR was significantly increased by 6.05-fold. The enhanced encapsulation efficiency and rapid encapsulation process proposed in this study could facilitate YCs-based microcarriers to encapsulate bioactive substances with higher bioaccessibility.

姜黄素 (CUR) 通过 pH 驱动方法被封装到酵母细胞 (YCs) 中，与传统扩散方法相比，负载能力增加了 5.04 倍，孵育时间减少了 43.63 倍。当 CUR 与 YCs 的质量比为 0.1 时，获得了最佳封装，负载能力和封装效率分别为 8.07% 和 80.66%。通过荧光显微镜、差示扫描量热法和热重分析证实了 CUR 封装到 YCs 中。傅里叶变换红外光谱和X射线衍射进一步证明，包封的CUR与细胞壁网络的甘露糖蛋白和β-葡聚糖通过疏水相互作用和无定形状态的氢键相互作用。体外的载有 YCs 的 CUR 的生物可及性显着增加了 6.05 倍。本研究提出的增强封装效率和快速封装过程可以促进基于YCs的微载体封装具有更高生物可及性的生物活性物质。