This study aimed to prepare and characterize antioxidative hydrolysate and its lipophilic peptides derived from mung bean protein. Our results indicated that the hydrolysate prepared with 120 min hydrolysis (MPH) possessed superb DPPH, ABTS radical scavenging activity and metal ion-chelating activity with IC₅₀ values of 11.19 ± 0.02, 18.06 ± 0.18 and 3.78 ± 0.59 µg/mL, respectively. MPH could also effectively retard lipid oxidation for oil-in-water emulsion (up to 50%) and linoleic acid (up to 94%), as well as for bulk oil. Interestingly, the lipophilic peptide fraction (MPHP) extracted from MPH could suppress the formation of primary (up to 50%) and secondary oxidation products (up to 90%) in bulk oil. Ten lipophilic peptides identified from MPHP using LC-MS/MS possessed high amounts of hydrophobic amino acids and histidine, which might favor peptides interacting and/or forming hydrogen bonds with lipid radicals. These findings provide a new perspective for lipophilic peptides in fortifying lipids as natural antioxidants.

本研究旨在制备和表征源自绿豆蛋白的抗氧化水解产物及其亲脂肽。我们的结果表明，120 min水解（MPH）制备的水解产物具有优异的DPPH、ABTS自由基清除活性和金属离子螯合活性，IC ₅₀值分别为 11.19 ± 0.02、18.06 ± 0.18 和 3.78 ± 0.59 µg/mL。MPH 还可以有效地延缓水包油乳液（高达 50%）和亚油酸（高达 94%）以及散装油的脂质氧化。有趣的是，从 MPH 中提取的亲脂肽级分 (MPHP) 可以抑制散装油中初级（高达 50%）和二级氧化产物（高达 90%）的形成。使用 LC-MS/MS 从 MPHP 中鉴定出的 10 种亲脂性肽具有大量的疏水性氨基酸和组氨酸，这可能有利于肽与脂质自由基相互作用和/或形成氢键。这些发现为亲脂肽在强化脂质作为天然抗氧化剂方面提供了新的视角。