In this study, for the first time, surface molecularly imprinted magnetic covalent organic frameworks (Fe₃O₄@COFs@MIPs) were combined with disposable screen-printed electrode (SPE) to construct a portable and on-site electrochemical sensor for the rapid detection of tetracycline (TC). The Fe₃O₄@COFs@MIPs, which was prepared by layer-by-layer modification method, had good magnetism and excellent adsorption ability. With the help of disposable SPE, equipped with a magnet, the electrode modification process was simplified and the detection efficiency was improved. Under optimal conditions, the fabricated electrochemical sensor exhibited linearity ranging from 1 × 10⁻¹⁰ to 1 × 10⁻⁴ g mL⁻¹. It had good selectivity, excellent reproducibility, desirable stability and remarkable applicability. The fabricated sensor was successfully applied to detect TC in real samples with satisfactory recoveries (96.15–106.20%). The detection strategy separated the recognition and adsorption process from the electrochemical detection process, providing a design idea for the application of COFs in the construction of high-efficiency molecularly imprinted electrochemical sensors.

本研究首次将表面分子印迹磁性共价有机骨架（Fe ₃ O ₄ @COFs@MIPs）与一次性丝网印刷电极（SPE）相结合，构建了一种便携式现场电化学传感器，用于快速四环素（TC）的检测。采用逐层改性方法制备的Fe ₃ O _{4 @COFs@MIPs具有良好的磁性和优异的吸附能力。}在一次性 SPE 的帮助下，配备磁铁，简化了电极修饰过程，提高了检测效率。在最佳条件下，制造的电化学传感器的线性范围为 1 × 10 ^-10至 1 × 10 ^-4 g mL^-1。它具有良好的选择性、优异的重现性、良好的稳定性和显着的适用性。制造的传感器成功应用于检测真实样品中的 TC，回收率令人满意（96.15-106.20%）。该检测策略将识别和吸附过程与电化学检测过程分离，为COFs在构建高效分子印迹电化学传感器中的应用提供了设计思路。