In this study, silver nanoparticle-coated polydopamine-copper phosphate hybrid nanoflowers (Ag-PDA-Cu₃(PO₄)₂ NFs) were synthesized and demonstrated to serve as efficient surface-enhanced Raman spectroscopy (SERS) probes for detecting thiol-containing molecules such as benzenethiol and thiocholine. Ag-PDA-Cu₃(PO₄)₂ NFs were fabricated by forming PDA-Cu₃(PO₄)₂ NFs through the nucleation of Cu₃(PO₄)₂ on the PDA surface via interaction between Cu²⁺ and the free amine groups of dopamine, followed by the anisotropic growth of Cu₃(PO₄)₂ crystals into flower-like microparticles. The NFs were then incubated with silver nitrate for in situ reduction to form Ag nanoparticles (NPs) on the surface of the NFs. The resulting hybrid NFs were demonstrated to act as SERS probes, sensitively detecting thiol-containing molecules, based on the successful interaction between Ag NPs and the thiol group (S-H) of the target molecules. The SERS intensities of benzenethiol and thiocholine at various concentrations were analyzed and found to yield superior limits of detection of 800 and 60 pM, respectively. Therefore, Ag-PDA-Cu₃(PO₄)₂ NFs could be excellent SERS probes for detecting various thiol-containing molecules, particularly to determine the status of patients exposed to thiol-containing pesticides.

在这项研究中，合成了银纳米颗粒包覆的聚多巴胺-磷酸铜杂化纳米花 (Ag-PDA-Cu ₃ (PO ₄ ) ₂ NFs)，并证明其可用作检测含硫醇的有效表面增强拉曼光谱 (SERS) 探针。苯硫醇和硫代胆碱等分子。Ag-PDA-Cu ₃ (PO ₄ ) ₂ NFs 是通过 Cu ₃ (PO ₄ ) _{2在 PDA 表面通过 Cu} ²⁺之间的相互作用形成 PDA-Cu ₃ (PO ₄ ) _{2 NFs 来制备的。}和多巴胺的游离胺基团，随后Cu ₃ (PO ₄ ) ₂晶体各向异性生长成花状微粒。然后将NFs与硝酸银一起温育以进行原位还原以在NFs表面上形成Ag纳米颗粒（NPs）。基于 Ag NPs 与目标分子的硫醇基团 (SH) 之间的成功相互作用，由此产生的混合 NFs 被证明可以作为 SERS 探针，灵敏地检测含硫醇分子。分析了不同浓度的苯硫醇和硫代胆碱的 SERS 强度，发现其检测限分别为 800 和 60 pM。因此，Ag-PDA-Cu ₃ (PO ₄ ) ₂NFs 可能是检测各种含硫醇分子的优秀 SERS 探针，特别是用于确定暴露于含硫醇农药的患者的状态。