The reliable detection of cancer-related biomarkers is essential for accurate diagnosis of cancers. SERS/electrochemical dual-mode sensing can provide a powerful tool to achieve more accurate detection, however, the in situ facile dual-mode detections have rarely been reported. Herein, a novel SERS/electrochemical dual-mode biosensor based on multi-functionalized molybdenum disulfide nanosheet (mF-MoS₂ NS) probes and SERS-active Ag nanorods (AgNRs) array electrode was proposed for in situ dual-mode detection of gastric cancer-related miR-106a. The AgNRs array coated by the mF-MoS₂ NSs shows significantly improved electrochemical and SERS stability in electrolyte solutions, and the “mF-MoS₂ NSs probes-miRNA-AgNRs array electrodes” sandwich structured SERS/electrochemical sensor was built for stable, in-situ dual-mode detection of miR-106a. The sensor shows good selectivity for differentiating miR-106a from the single-base mismatched nucleic acid, and possesses linear calibration curves of both SERS and electrochemical sensing modes with the limit of detections of 67.44 fM and 248.01 fM, respectively. The reliable assay of miR-106a in human serum indicates that the proposed SERS/electrochemical dual-mode sensor is suitable for detecting complex samples and can be a potential high-sensitive and reliable tool for clinical diagnosis of early cancers.

癌症相关生物标志物的可靠检测对于准确诊断癌症至关重要。SERS/电化学双模传感可以提供一种强大的工具来实现更准确的检测，然而，原位简便的双模检测很少被报道。在此，提出了一种基于多功能二硫化钼纳米片（mF-MoS ₂ NS）探针和SERS活性银纳米棒（AgNRs）阵列电极的新型SERS/电化学双模生物传感器，用于原位双模检测胃癌。相关的 miR-106a。_{由 mF-MoS 2} NSs包覆的 AgNRs 阵列在电解质溶液中显示出显着改善的电化学和 SERS 稳定性，而“mF-MoS ₂NSs probes-miRNA-AgNRs array electrode”的夹层结构SERS/电化学传感器是为稳定、原位双模式检测miR-106a而构建的。该传感器对区分 miR-106a 和单碱基错配核酸具有良好的选择性，具有 SERS 和电化学传感模式的线性校准曲线，检测限分别为 67.44 fM 和 248.01 fM。人血清中 miR-106a 的可靠测定表明，所提出的 SERS/电化学双模传感器适用于检测复杂样品，可以成为早期癌症临床诊断的潜在高灵敏度和可靠工具。