Biobased chemical furfuryl alcohol is an important furfural-upgrading compound, which is mainly used to prepare fruit acids, resins, additives, polymers, fibers, and rocket fuels. In this study, one sustainable way for valorisation of biomass into furfuryl alcohol was developed via chemoenzymatic conversion in one-pot manner. Deep eutectic solvent (DES) CA:Betaine was prepared by mixing betaine and citric acid (CA). Using CA:Betaine (10 wt%) as catalyst and reaction medium, the carbohydrates (glucan plus xylan) in corncob (60 g/L) were firstly catalyzed to produce 74.6 mM furfural, 96.5 mM formic acid and 40.3 mM glucose at 170 °C for 30 min in CA:Betaine-water (10:90, wt:wt). Furthermore, co-expression of formate dehydrogenase (FDH) and NADPH-dependent aldehyde reductase (SsCR) from Sporidiobolus salmonicolor in one E. coli was a good strategy for the efficient biosynthesis of furfuryl alcohol. Within 2 h, E. coli SF21 efficiently converted corncob-derived furfural (74.6 mM) to furfuryl alcohol in a yield of 95.8 % by using corncob-derived formate and glucose as co-substrates. The furfuryl alcohol productivity was obtained at 0.117 g furfuryl alcohol/g corncob (0.343 g furfuryl alcohol/g xylan). Overall, this study provided a novel chemoenzymatic strategy for catalysis of biomass into furfuryl alcohol in CA:Betaine-water.

生物基化工糠醇是一种重要的糠醛改质化合物，主要用于制备果酸、树脂、添加剂、聚合物、纤维和火箭燃料等。在这项研究中，一种可持续的生物质转化为糠醇的方法是通过一锅法的化学酶转化开发的。低共熔溶剂 (DES) CA：甜菜碱是通过混合甜菜碱和柠檬酸 (CA) 制备的。以CA:甜菜碱（10 wt%）为催化剂和反应介质，首先在170°催化玉米芯（60 g/L）中的碳水化合物（葡聚糖加木聚糖）生成74.6 mM糠醛、96.5 mM甲酸和40.3 mM葡萄糖C 在 CA: 甜菜碱水 (10:90, wt:wt) 中 30 分钟。此外，甲酸脱氢酶 (FDH) 和 NADPH 依赖性醛还原酶 (SsCR) 的共表达一种大肠杆菌中的Sporidiobolus Salmonicolor是糠醇有效生物合成的良好策略。在 2 小时内，大肠杆菌SF21 使用玉米芯衍生的甲酸盐和葡萄糖作为共底物，有效地将玉米芯衍生的糠醛 (74.6 mM) 转化为糠醇，收率为 95.8%。在 0.117 g 糠醇/g 玉米芯（0.343 g 糠醇/g 木聚糖）下获得糠醇产率。总体而言，本研究提供了一种新的化学酶促策略，用于在 CA:甜菜碱-水中将生物质催化为糠醇。