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Co-production of xylose, lignin, and ethanol from eucalyptus through a choline chloride-formic acid pretreatment
Bioresource Technology ( IF 9.7 ) Pub Date : 2022-06-17 , DOI: 10.1016/j.biortech.2022.127502
Xiaohua Zhang 1 , Yaohong Zhou 1 , Wanming Xiong 1 , Weiqi Wei 2 , Weikun Jiang 3
Affiliation  

A choline chloride-formic acid (ChCl-FA) pretreatment followed by enzymatic hydrolysis and fermentation were developed in this work for co-produce bioethanol, xylose, and lignin from eucalyptus. Results showed that ChCl-FA pretreatment can simultaneously degrade the xylan (∼95.2%) and lignin (∼74.4%) in eucalyptus, and obtained the pretreated eucalyptus having high glucan content and a numbers of cracks and holes, which was conducive to follow-up cellulase attacking. The hydrolysis experiments showed the maximum yield of glucose of 100 g eucalyptus was 35.3 g, which was equivalent to 90.3% of glucan in eucalyptus feedstock. The fermentation of enzymatic hydrolysate finally achieved the ethanol yield of 16.5 g, which corresponded to 74.5% theoretical ethanol yield from initial glucan in eucalyptus. In addition, 12.1 g xylose and 23.9 g lignin also could be obtained in pretreated liquid or/and hydrolysis residue, which represented for 61.4% xylan and 80.7% lignin in eucalyptus feedstock, respectively.

中文翻译:


通过氯化胆碱-甲酸预处理从桉树中联产木糖、木质素和乙醇



这项工作开发了一种氯化胆碱-甲酸 (ChCl-FA) 预处理,然后进行酶水解和发酵的方法,用于从桉树中联产生物乙醇、木糖和木质素。结果表明,ChCl-FA预处理可以同时降解桉树中的木聚糖(~95.2%)和木质素(~74.4%),得到的预处理桉树葡聚糖含量高,且存在大量裂纹和孔洞,有利于后续处理。纤维素酶攻击。水解实验表明,100 g桉树的葡萄糖最大产量为35.3 g,相当于桉树原料中葡聚糖的90.3%。酶解产物发酵最终获得16.5 g的乙醇产量,相当于桉树中初始葡聚糖理论乙醇产量的74.5%。此外,在预处理的液体或/和水解残渣中还可以获得12.1 g木糖和23.9 g木质素,分别代表桉树原料中的木聚糖和木质素的61.4%和80.7%。
更新日期:2022-06-17
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