This work explored the microbial mechanisms for the improvement of composting efficiency driven by thermotolerant lignin-degrading bacterium Aneurinibacillus sp. LD3 (LD3). Results showed that LD3 inoculant prolonged the thermophilic period by 4 days, improved the final content of humic acid, total phosphorus (TP), nitrogen, potassium and seed germination index. Inoculating LD3 enhanced the relative abundance of thermotolerant and phosphate-solubilizing microbes including the phyla of Proteobacteria, Bacteroidota, Firmicutes, and Actinobacteriota, and the genus of Bacillus, Thermoactinomyces, and Pseudomonas. Metabolic function analysis showed that sequences involved in carbohydrate and amino acid metabolism were boosted, while sequences associated with human disease were reduced after inoculating LD3. Spearman correlation analysis revealed that Aneurinibacillus has a significant positive correlation with temperature, TP, Bacillus, and Thermoactinomyces. This study provides useful information for understanding the microbial mechanisms of LD3 promoting composting efficiency, and reveals the tremendous potential of LD3 in the resource utilization of organic solid wastes.

这项工作探索了由耐热木质素降解细菌Aneurinibacillus sp驱动的提高堆肥效率的微生物机制。LD3 (LD3)。结果表明，LD3孕育剂使嗜热期延长4天，提高了最终腐殖酸含量、总磷（TP）、氮、钾和种子发芽指数。接种 LD3 可提高耐热和解磷微生物的相对丰度，包括变形菌门、拟杆菌门、厚壁菌门和放线菌门，以及芽孢杆菌属、嗜热放线菌属和假单胞菌属. 代谢功能分析表明，在接种 LD3 后，参与碳水化合物和氨基酸代谢的序列增加，而与人类疾病相关的序列减少。Spearman相关分析表明，无神经芽孢杆菌与温度、TP、芽孢杆菌和嗜热放线菌呈显着正相关。该研究为了解LD3促进堆肥效率的微生物机制提供了有用的信息，并揭示了LD3在有机固体废物资源化利用方面的巨大潜力。