The packing structure of intercellular lipids in skin forms an important biological barrier but little is known about the changes in packing structure caused by surfactant-induced lipid effluence. In this study, to examine the effect of the decrease in lipids in the lipid model (LM) on the packing structure, a single component was reduced from stratum corneum intercellular LM comprising ceramide ADS, cholesterol, and palmitic acid. The packing structure was assessed using differential scanning calorimetry, Raman spectroscopy, and powder X-ray diffraction. Surfactants were applied to LM to investigate lipid elution and changes in the packing structure. The results showed that the decrease in cholesterol caused disorder in the lipid structure and the amount of cholesterol was strongly inversely correlated with the hexagonal structure ratio (R_Hex/Ort) in the packing structure. Furthermore, the R_Hex/Ort values were highly correlated with the amount of lipids eluted by surfactants, making it possible to measure lipid elution using R_Hex/Ort values. The methods used in this study are a useful alternative to native intercellular lipids for elucidating mechanisms underlying skin irritation due to surfactants.

皮肤细胞间脂质的堆积结构形成了重要的生物屏障，但对表面活性剂诱导的脂质流出引起的堆积结构变化知之甚少。在这项研究中，为了检查脂质模型 (LM) 中脂质减少对包装结构的影响，从角质层细胞间 LM 中减少了一种成分，包括神经酰胺 ADS、胆固醇和棕榈酸。使用差示扫描量热法、拉曼光谱和粉末 X 射线衍射评估填料结构。将表面活性剂应用于 LM 以研究脂质洗脱和包装结构的变化。结果表明，胆固醇的降低导致脂质结构紊乱，胆固醇的量与六角形结构比呈强负相关（R_Hex/Ort ) 在包装结构中。此外，R _Hex/Ort值与表面活性剂洗脱的脂质量高度相关，因此可以使用 R _Hex/Ort值测量脂质洗脱。本研究中使用的方法是天然细胞间脂质的有用替代方法，用于阐明由表面活性剂引起的皮肤刺激机制。