Antimony (Sb) and arsenic (As) are chemical analogs, but their behaviors in plants are different. To investigate the Sb uptake, translocation and speciation in As-hyperaccumulator P. cretica, a hydroponic experiment was conducted. In this study, P. cretica was exposed to 0.2-strength Hoagland nutrient solution, which contained 0.5 or 5 mg/L antimonite (SbIII) or antimonate (SbV). After 14 d exposure, P. cretica took up 1.4–2.8 times more SbIII than SbV. Since P. cretica was unable to translocate Sb, its roots accumulated >97% Sb with the highest at 7965 mg/kg. In both SbIII and SbV treatments, SbIII was the predominant species in P. cretica, with 90–100% and 46–100% SbIII in the roots. As the first barrier against Sb to enter plant cells, more Sb was accumulated in cell wall than cytosol or organelles. The results suggest that P. cretica may detoxify Sb by reducing SbV to SbIII and immobilizing it in root cell walls. Besides, the presence of SbIII significantly reduced the concentrations of dissolved organic C including organic acids in P. cretica root exudates. Further, increasing Sb levels promoted P accumulation in the plant, especially in the fronds, which may help P. cretica growth. The information from this study shed light on metabolic transformation of Sb in As-hyperaccumulators P. cretica, which helps to better understand Sb uptake and detoxification by plants.

锑 (Sb) 和砷 (As) 是化学类似物，但它们在植物中的行为不同。为了研究 As-hypercumulator P. cretica中 Sb 的吸收、转运和形态形成，进行了水培实验。在这项研究中，P. cretica暴露于 0.2 浓度的 Hoagland 营养液中，其中含有 0.5 或 5 mg/L 锑酸盐 (SbIII) 或锑酸盐 (SbV)。暴露 14 天后，P. cretica吸收的 SbIII 是 SbV 的 1.4-2.8 倍。由于P. cretica无法转运 Sb，其根系积累的 Sb > 97%，最高为 7965 mg/kg。在 SbIII 和 SbV 处理中，SbIII 是P. cretica中的主要物种，根中有 90-100% 和 46-100% SbIII。作为 Sb 进入植物细胞的第一道屏障，在细胞壁中积累的 Sb 多于胞质溶胶或细胞器。结果表明，P. cretica可以通过将 SbV 还原为 SbIII 并将其固定在根细胞壁中来对 Sb 进行解毒。此外， SbIII的存在显着降低了白杨根分泌物中溶解的有机碳（包括有机酸）的浓度。此外，增加 Sb 水平促进植物中磷的积累，尤其是在叶中，这可能有助于P. cretica的生长。这项研究的信息揭示了 Sb 在 As 超富集植物P. cretica中的代谢转化，这有助于更好地了解植物对 Sb 的吸收和解毒。