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Cationic polyelectrolyte as powerful capture molecule in aptamer-based chromatographic strip for rapid visual detection of paraquat residue in agricultural products
Sensors and Actuators B: Chemical ( IF 8.0 ) Pub Date : 2022-06-17 , DOI: 10.1016/j.snb.2022.132237
Yuanyuan Liu , Bangyan Liu , Lian Xia , Hongyan Yu , Qiaodie Wang , Yuangen Wu

Although methods based on mass spectrometry or immunoassays can meet the demands of paraquat detection, their application is limited by a lack of convenience or stability. Herein, a facile visual aptamer-based chromatographic strip (CS) was fabricated for the rapid paraquat detection using the cationic polyelectrolyte poly(diallyl dimethyl ammonium chloride) (PDDA) as a powerful capture molecule at test-line (T-line). The PDDA could capture the gold nanoparticles (AuNPs) through strong electrostatic interactions for visual detection. The proposed CS showed an excellent response for paraquat in the range of 20–150 μg·L−1, and the limit of detection (LOD) was as low as 4.28 μg·L−1. This detection method has good stability and specific selectivity, and can visually determine paraquat residues more than 30 μg·L−1 by naked eye within 3 min. The results demonstrated that the prepared CS based on aptamer and cationic polyelectrolyte is an accurate and practicable tool for rapid analysis of pesticide residues.



中文翻译:

阳离子聚电解质作为适体色谱条中的强大捕获分子,用于快速目测农产品中的百草枯残留

虽然基于质谱或免疫分析的方法可以满足百草枯检测的需求,但它们的应用受限于缺乏便利性或稳定性。在此,使用阳离子聚电解质聚(二烯丙基二甲基氯化铵)(PDDA)作为测试线(T线)的强大捕获分子,制造了一种简便的基于适配体的可视化色谱条(CS),用于快速检测百草枯。PDDA 可以通过强静电相互作用捕获金纳米颗粒 (AuNPs) 以进行视觉检测。所提出的 CS 在 20-150 μg·L -1范围内对百草枯表现出优异的响应,检测限 (LOD) 低至 4.28 μg·L -1. 该检测方法具有良好的稳定性和特异性选择性,可在3 min内用肉眼目测30 μg·L -1以上的百草枯残留量。结果表明,基于适配体和阳离子聚电解质制备的CS是一种准确、实用的农药残留快速分析工具。

更新日期:2022-06-22
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