Compost tea was alternatives of chemical pesticide for green agriculture, but there were no reports about antibiotic resistance genes (ARGs) in compost tea. This study investigated the effect of livestock manures, sewage sludge, their composting products and liquid fermentation on ARGs, mobile genetic elements (MGEs), metal resistance genes (MRGs) and antimicrobial properties of various compost tea. The results showed aerobic liquid fermentation reduced ARGs by 65.93 % and 45.20 % in the compost tea of chicken manure and sludge, enriched ARGs by 8.57 % and 37.41 % in the compost tea of pig manure and bovine manure, and increased MGEs and MRGs by 1.25 × 10^-5-5.53 × 10^-3 and 2.03 × 10^-5-2.03 × 10^-3 in the four compost tea. The correlation coefficient of tetracycline and sulfonamide resistance genes between compost product and compost tea were 0.98 and 0.91. aadA2–02, sul2 and tetX abundant in the compost tea were positively correlated with MGEs and MRGs. Furthermore, liquid fermentation enriched the potential host of tetracycline and vancomycin resistance genes. Tetracycline resistance genes occupied 62.7 % of total ARGs in the compost tea. Alcaligenes and Bacillus enriched by 0.78–39.31 % in the four compost tea, which metabolites had high antimicrobial activity. The potential host of ARGs accounted for 42.1 % bacteria abundance in the four compost tea.

堆肥茶是绿色农业化学农药的替代品，但没有关于堆肥茶中抗生素抗性基因（ARGs）的报道。本研究调查了牲畜粪便、污水污泥、其堆肥产品和液体发酵对各种堆肥茶的 ARGs、移动遗传元件 (MGEs)、金属抗性基因 (MRGs) 和抗菌特性的影响。结果表明，有氧液体发酵使鸡粪和污泥堆肥茶中的ARGs降低了65.93%和45.20%，猪粪和牛粪堆肥茶中的ARGs富集了8.57%和37.41%，MGEs和MRGs分别提高了1.25%。 × 10 ^-5 -5.53 × 10 ^-3和 2.03 × 10 ^-5 -2.03 × 10 ^-3在四堆肥茶。堆肥产品与堆肥茶的四环素和磺胺类抗性基因相关系数分别为0.98和0.91。堆肥茶中丰富的 aadA2-02、sul2 和 tetX 与 MGEs 和 MRGs 呈正相关。此外，液体发酵富集了四环素和万古霉素抗性基因的潜在宿主。四环素抗性基因占堆肥茶中总 ARGs 的 62.7%。四种堆肥茶中产碱菌和芽孢杆菌的含量分别为0.78-39.31 %，其代谢物具有较高的抗菌活性。在四种堆肥茶中，ARGs 的潜在宿主占细菌丰度的 42.1%。