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Decellularized adipose tissue matrix-coated and simvastatin-loaded hydroxyapatite microspheres for bone regeneration
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-06-16 , DOI: 10.1002/bit.28154 Merve G Kesim 1 , Caner Durucan 2, 3 , Deniz Atila 3, 4 , Dilek Keskin 1, 3, 4 , Ayşen Tezcaner 1, 3, 4
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2022-06-16 , DOI: 10.1002/bit.28154 Merve G Kesim 1 , Caner Durucan 2, 3 , Deniz Atila 3, 4 , Dilek Keskin 1, 3, 4 , Ayşen Tezcaner 1, 3, 4
Affiliation
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Simvastatin (SIM)-loaded and human decellularized adipose tissue (DAT)-coated porous hydroxyapatite (HAp) microspheres were developed for the first time to investigate their potential on bone regeneration. Microspheres were loaded with SIM and then coated with DAT for modifying SIM release and improving their biological response. HAp microspheres were prepared by water-in-oil emulsion method using camphene (C10H16) as porogen followed by camphene removal by freeze-drying and sintering at 1200°C for 3 h. Sintered HAp microspheres with an average particle size of ~400 µm were porous and spherical in shape. Microspheres were incubated with 1, 2.5, and 5 mg/ml SIM stock solutions for drug loading, and drug loading was determined as 7.5 ± 0.79, 20.41 ± 1.93, and 46.26 ± 0.29 µg SIM/mg microspheres, respectively. SIM loading increased with the increase of the initial SIM loading amount. Faster SIM release was observed in DAT-coated microspheres compared to bare counterparts. Higher SaoS-2 cell attachment and proliferation were observed on DAT-coated microspheres. Significantly higher alkaline phosphatase activity of SaoS-2 cells was observed on DAT-coated microspheres containing 0.01 mg/ml SIM than all other groups (p < 0.01). DAT-coated microspheres loaded with SIM at low doses hold promise for bone tissue engineering applications.
中文翻译：
脱细胞脂肪组织基质涂层和辛伐他汀负载羟基磷灰石微球用于骨再生
首次开发了负载辛伐他汀 (SIM) 和人脱细胞脂肪组织 (DAT) 涂层的多孔羟基磷灰石 (HAp) 微球，以研究其对骨再生的潜力。微球上装有 SIM，然后用 DAT 包被，以改变 SIM 的释放并改善其生物反应。采用莰烯(C 10 H 16 ) 油包水乳液法制备HAp微球) 作为致孔剂，然后通过冷冻干燥和在 1200°C 下烧结 3 小时去除莰烯。平均粒径约为 400 µm 的烧结 HAp 微球是多孔和球形的。微球与 1、2.5 和 5 mg/ml SIM 原液一起孵育用于载药，载药量分别确定为 7.5 ± 0.79、20.41 ± 1.93 和 46.26 ± 0.29 µg SIM/mg 微球。SIM 加载随着初始 SIM 加载量的增加而增加。与裸对应物相比，在 DAT 涂层微球中观察到更快的 SIM 释放。在 DAT 包被的微球上观察到更高的 SaoS-2 细胞附着和增殖。在含有 0.01 mg/ml SIM 的 DAT 包被微球上观察到 SaoS-2 细胞的碱性磷酸酶活性显着高于所有其他组（p < 0.01)。以低剂量装载 SIM 的 DAT 涂层微球有望用于骨组织工程应用。
更新日期：2022-06-16
中文翻译：
脱细胞脂肪组织基质涂层和辛伐他汀负载羟基磷灰石微球用于骨再生
首次开发了负载辛伐他汀 (SIM) 和人脱细胞脂肪组织 (DAT) 涂层的多孔羟基磷灰石 (HAp) 微球，以研究其对骨再生的潜力。微球上装有 SIM，然后用 DAT 包被，以改变 SIM 的释放并改善其生物反应。采用莰烯(C 10 H 16 ) 油包水乳液法制备HAp微球) 作为致孔剂，然后通过冷冻干燥和在 1200°C 下烧结 3 小时去除莰烯。平均粒径约为 400 µm 的烧结 HAp 微球是多孔和球形的。微球与 1、2.5 和 5 mg/ml SIM 原液一起孵育用于载药，载药量分别确定为 7.5 ± 0.79、20.41 ± 1.93 和 46.26 ± 0.29 µg SIM/mg 微球。SIM 加载随着初始 SIM 加载量的增加而增加。与裸对应物相比，在 DAT 涂层微球中观察到更快的 SIM 释放。在 DAT 包被的微球上观察到更高的 SaoS-2 细胞附着和增殖。在含有 0.01 mg/ml SIM 的 DAT 包被微球上观察到 SaoS-2 细胞的碱性磷酸酶活性显着高于所有其他组（p < 0.01)。以低剂量装载 SIM 的 DAT 涂层微球有望用于骨组织工程应用。
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