An aminooxy click chemistry (AOCC) strategy was used to synthesize nucleoside building blocks for incorporation during solid-support synthesis of oligonucleotides to enable bis-homo and bis-hetero conjugation of various biologically relevant ligands. The bis-homo aminooxy conjugation leads to bivalent ligand presentation, whereas the bis-hetero conjugation allows the placement of different ligands with either the same or different chemical linkages. This facile synthetic methodology allows introduction of two different ligands with different biological functions simultaneously.

中文翻译：

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氨基氧点击化学作为双同型和双杂配体与核酸缀合的工具

氨基氧点击化学 (AOCC) 策略用于合成核苷构建块，用于在寡核苷酸的固相支持合成过程中掺入，以实现各种生物学相关配体的双同型和双杂共轭。双高氨基氧基共轭导致二价配体呈递，而双杂共轭允许放置具有相同或不同化学键的不同配体。这种简便的合成方法允许同时引入具有不同生物学功能的两种不同配体。