The influence of temperature (25 and 32 °C) on the negative effects of the herbicide tebuthiuron (TBU, 0, 10, 50 and 200 ng.L-1, 16 days) on thyroid function and metamorphosis of Lithobates catesbeianus tadpoles was evaluated. Metamorphosis was accelerated by TBU exposure at 25 ºC, but delayed at 32 ºC with considerable losses of body mass. T3 and T4 levels were not altered. The highest TBU concentrarion at 25 ºC increased TR β and DIO3 transcript levels, which is consistent with development acceleration in tadpoles. At 32 ºC TR β transcript levels were lower than the values recorded at 25 ºC, and those tadpoles exposed to the highest TBU concentration presented increased diameter of thyroid follicles compared to controls at same temperature. This study evidences that TBU at environmentally realistic concentrations is able to disrupt thyroidogenesis in bullfrog tadpoles, impairing their development. These effects are influenced by temperature.

评估了温度（25 和 32 °C）对除草剂丁硫脲（TBU，0、10、50 和 200 ng.L-1、16 天）对Lithobates catesbeianus蝌蚪甲状腺功能和变态的负面影响的影响。在 25 ºC 时暴露于 TBU 会加速变态，但在 32 ºC 时会延迟，导致体重显着下降。T3 和 T4 水平没有改变。25 ºC 时的最高 TBU 浓度增加了TR β和DIO3转录水平，这与蝌蚪的发育加速一致。在 32 ºC TR β转录水平低于 25 ºC 时记录的值，与相同温度下的对照组相比，暴露于最高 TBU 浓度的蝌蚪表现出甲状腺滤泡直径增加。这项研究证明，环境现实浓度的 TBU 能够破坏牛蛙蝌蚪的甲状腺发生，损害它们的发育。这些影响受温度影响。