Abstract

Objective

IGF-axis and mitotic capacity of cells play a vital role in fetal growth. We compared IGF1, IGF2, and IGFBP3 protein levels, mitotic indices, IGFR1 and IGFR2 mRNA expression in appropriate for gestational age (AGA) and small for gestational age (SGA) neonates of Indian women.

Methods

Cord blood (n = 80) and placental samples (n = 40) were collected from AGA and SGA neonates. Plasma IGF1, IGF2, and IGFBP3 proteins were measured by ELISA. IGFR1 and IGFR2 mRNA expression in the placenta were analyzed by qRT-PCR. Cord blood was cultured in vitro and mitotic index was obtained.

Results

IGF1 (p = 1), and IGF2 (p = 0.69) protein levels did not differ, whereas IGFBP3 (p = 0.02) was significantly less in SGA compared to AGA neonates. Down-regulation of IGFR1 (3.9-folds) and IGFR2 (2.8-folds) mRNA and reduced mitotic index of lymphocytes was observed in SGA (p = 0.001) compared to AGA neonates.

Conclusion

Our results showed that, SGA neonates displayed down-regulated IGFR1 and IGFR2 mRNA, decreased IGFBP3 protein and mitotic index.

中文翻译：

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胰岛素样生长因子轴和有丝分裂指数在南印度小于胎龄儿中的作用

摘要

客观的

IGF 轴和细胞的有丝分裂能力在胎儿生长中起着至关重要的作用。我们比较了印度妇女适龄 (AGA) 和小于胎龄 (SGA) 新生儿的IGF1、IGF2 和 IGFBP3 蛋白水平、有丝分裂指数、IGFR1和IGFR2 mRNA 表达。

方法

 从 AGA 和 SGA 新生儿收集脐带血 ( n  = 80) 和胎盘样本 ( n = 40)。通过 ELISA 测量血浆 IGF1、IGF2 和 IGFBP3 蛋白。通过 qRT-PCR 分析胎盘中的IGFR1和IGFR2 mRNA 表达。脐带血体外培养，获得有丝分裂指数。

结果

IGF1 ( p  = 1) 和 IGF2 ( p  = 0.69) 蛋白水平没有差异，而 IGFBP3 ( p  = 0.02) 在 SGA 新生儿中明显低于 AGA 新生儿。 与 AGA 新生儿相比，在 SGA ( p = 0.001) 中观察到IGFR1（3.9 倍）和IGFR2（2.8 倍）mRNA的下调和淋巴细胞有丝分裂指数降低。

结论

我们的结果表明，SGA 新生儿表现出下调的IGFR1和IGFR2 mRNA，降低的 IGFBP3 蛋白和有丝分裂指数。