Liquid–liquid phase separation (LLPS) is an important phenomenon in biology, and it is desirable to develop quantitative methods to analyze protein droplets generated by LLPS. This study quantified the change in protein concentration in a droplet in label-free and single-droplet conditions using Raman imaging and the Raman band of water as an intensity standard. Small changes in the protein concentration with variations in pH and salt concentration were observed, and it was shown that the concentration in the droplet decreases as the conditions become less favorable for droplet formation. The effect of exposure to 1,6-hexanediol was also examined, and this additive was found to decrease the protein concentration in the droplet. A model can be proposed in which the addition of 1,6-hexanediol reduces the protein concentration in the droplet, and the droplet disappears when the concentration falls below a certain threshold value.

中文翻译：

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单个液滴内融合肉瘤的低复杂度域的浓度量化和溶液参数的影响

液-液相分离 (LLPS) 是生物学中的重要现象，需要开发定量方法来分析 LLPS 产生的蛋白液滴。本研究使用拉曼成像和水的拉曼带作为强度标准，量化了无标记和单液滴条件下液滴中蛋白质浓度的变化。观察到蛋白质浓度随 pH 值和盐浓度的变化而发生微小变化，并且表明随着条件变得不利于液滴形成，液滴中的浓度降低。还检查了暴露于 1,6-己二醇的影响，发现这种添加剂会降低液滴中的蛋白质浓度。可以提出一个模型，其中添加 1,6-己二醇会降低液滴中的蛋白质浓度，