A nanozyme with 2D/1D heterostructure has been fabricated by the in-situ growth of molybdenum disulfide nanosheets (MoS₂ NSs) onto single-walled carbon nanotubes (SWCNTs). It was discovered that the so-obtained SWCNTs@MoS₂ nanozyme could exhibit greatly improved peroxidase-like catalysis, due to that the formed 2D/1D interfacial coupling in the heterostructure might provide more active sites and exhibit enhanced charge transferring during the catalytic reactions, as confirmed by the X-ray photoelectron spectroscopy, photoluminescence, electrochemical impedance spectra and radical capturing experiments. Furthermore, the catalysis of the developed nanozyme could be selectively inhibited by glutathione (GSH) through the competitive consumption of hydroxyl radicals with enzyme substrate in the catalytic reaction system. A SWCNTs@MoS₂ catalysis-based colorimetric strategy was further proposed for the quantitative analysis of GSH with the concentrations linearly ranging from 0.01 to 1000.0 μM. Besides, the feasibility of the developed colorimetric method was evaluated by monitoring GSH separately in the extractions from hela cells and human serum, promising the extensive applications for monitoring various biological species like GSH in the clinical laboratory. Importantly, such a fabrication route for nanozyme with 2D/1D heterostructure may pave the way towards the wide applications for designing various nanzymes with improved catalysis.

通过在单壁碳纳米管 (SWCNT) 上原位生长二硫化钼纳米片 (MoS ₂ NSs)制备了具有 2D/1D 异质结构的纳米酶。发现如此获得的 SWCNTs@MoS ₂X 射线光电子能谱、光致发光、电化学阻抗谱和自由基捕获实验。此外，通过在催化反应系统中与酶底物竞争消耗羟基自由基，谷胱甘肽（GSH）可以选择性地抑制所开发的纳米酶的催化作用。A SWCNTs@MoS ₂进一步提出了基于催化的比色策略用于 GSH 的定量分析，其浓度线性范围为 0.01 至 1000.0 μM。此外，通过在hela细胞和人血清提取物中分别监测GSH，评估了所开发的比色法的可行性，有望在临床实验室监测GSH等各种生物物种的广泛应用。重要的是，这种具有 2D/1D 异质结构的纳米酶的制造路线可能为设计具有改进催化作用的各种纳米酶的广泛应用铺平道路。