A high throught methododology based on a green extraction technique, µSPEed®, followed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has been proposed for the analysis of atropine and scopolamine in tea and herbal tea infusions. For this, a digiVOL® Digital Syringe was used with different sorbents and working conditions to obtain a fast and efficient µSPEed® extraction. The best performance was achieved with a PS/DVB sorbent phase, sample loading of 5 × 500 µL and elution with 2 × 100 µL aliquots of methanol. The strategy based on µSPEed® followed by HPLC-MS/MS was validated, attaining quantitation limits lower than 0.15 ng mL⁻¹ and recoveries between 94 and 106% for both analytes and applied to seventeen tea and herbal tea infusions. Fourteen infusions showed contamination with one or both analytes above the maximum content legislated (sum of atropine and scopolamine < 0.2 ng mL⁻¹).

已经提出了一种基于绿色提取技术 µSPEed® 的高通量方法，然后采用高效液相色谱-串联质谱 (HPLC-MS/MS) 分析茶和凉茶浸液中的阿托品和东莨菪碱。为此，digiVOL® 数字注射器与不同的吸附剂和工作条件一起使用，以获得快速高效的 µSPEed® 萃取。使用 PS/DVB 吸附相、上样量为 5 × 500 µL 并用 2 × 100 µL 等份甲醇洗脱时，性能最佳。验证了基于 µSPEed® 和 HPLC-MS/MS 的策略，定量限低于 0.15 ng mL ^-1两种分析物的回收率在 94% 到 106% 之间，并应用于 17 种茶和凉茶浸液。十四次输液显示一种或两种分析物的污染高于法定最大含量（阿托品和东莨菪碱的总和 < 0.2 ng mL ^-1）。