Overexpression of Multiple Cytochrome P450 Genes Conferring Clothianidin Resistance in Bradysia odoriphaga,Journal of Agricultural and Food Chemistry

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Overexpression of Multiple Cytochrome P450 Genes Conferring Clothianidin Resistance in Bradysia odoriphaga
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-16 , DOI: 10.1021/acs.jafc.2c01315
Chunni Zhang ₁ , Shaokai Du ₁ , Ruifang Liu ₁ , Wu Dai ₁

Affiliation

Cytochrome P450 monooxygenases (P450s) play important roles in the detoxification metabolism of xenobiotics and are involved in the resistance of insects to many insecticides. In this study, piperonyl butoxide (PBO), an inhibitor of P450 enzyme activity, significantly increased the toxicity of clothianidin in the clothianidin-resistant (CL-R) population of Bradysia odoriphaga. The enzyme activity of P450 in the CL-R population was significantly higher than that in the SS population. Furthermore, four P450 genes were found to be significantly overexpressed in the CL-R population. Tissue-specific expression analysis indicates that CYP9J57, CYP3828A1, CYP6SX1, and CYP6QE1 were most highly expressed in the midgut and/or Malpighian tubules. After exposure to LC₃₀ of clothianidin, the expression levels of the four P450 genes were significantly upregulated. The RNAi-mediated knockdown of CYP9J57, CYP3828A1, and CYP6QE1 significantly increased the susceptibility of B. odoriphaga to clothianidin. These results suggest that P450 genes are involved in clothianidin resistance in B. odoriphaga. This provides a better understanding of P450-mediated clothianidin resistance in B. odoriphaga and will contribute to the management of insect resistance to insecticides.

中文翻译：

在 Bradysia odoriphaga 中过度表达赋予氯噻菌胺抗性的多个细胞色素 P450 基因

细胞色素 P450 单加氧酶 (P450s) 在外源性物质的解毒代谢中发挥重要作用，并参与昆虫对多种杀虫剂的抗性。在这项研究中，P450 酶活性抑制剂胡椒基丁醇 (PBO) 显着增加了噻虫胺抗性 (CL-R) 种群中的噻虫胺毒性。CL-R群体中P450的酶活性显着高于SS群体。此外，发现四个 P450 基因在 CL-R 群体中显着过表达。组织特异性表达分析表明CYP9J57、CYP3828A1、CYP6SX1和CYP6QE1在中肠和/或马尔皮基小管中表达最高。暴露于噻虫胺的 LC ₃₀后，四种 P450 基因的表达水平显着上调。RNAi 介导的CYP9J57、CYP3828A1和CYP6QE1敲低显着增加了B. odoriphaga对噻虫胺的敏感性。这些结果表明，P450 基因与B. odoriphaga的噻虫胺抗性有关。这有助于更好地了解B. odoriphaga中 P450 介导的噻虫胺抗性，并将有助于管理昆虫对杀虫剂的抗性。

更新日期：2022-06-16

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