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High-Efficiency CdSe Quantum Dots/Fe3O4@MoS2/S2O82– Electrochemiluminescence System Based on a Microfluidic Analysis Platform for the Sensitive Detection of Neuron-Specific Enolase
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-06-16 , DOI: 10.1021/acs.analchem.2c01868 Tao Feng 1 , Xianzhen Song 1 , Yu Du 1 , Yu Bai 1 , Xiang Ren 1 , HongMin Ma 1 , Dan Wu 1 , YuYang Li 1 , Qin Wei 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-06-16 , DOI: 10.1021/acs.analchem.2c01868 Tao Feng 1 , Xianzhen Song 1 , Yu Du 1 , Yu Bai 1 , Xiang Ren 1 , HongMin Ma 1 , Dan Wu 1 , YuYang Li 1 , Qin Wei 1, 2
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In this work, based on electrochemiluminescence (ECL) technology and self-assembled portable disease detection chips, a bioactivity-maintained sensing platform was developed for the quantitative detection of neuron-specific enolase. First, we prepared Fe3O4@MoS2 nanocomposites as an efficient catalyst to accelerate the reduction of persulfate (S2O82–). Specifically, abundant sulfate radicals (SO4•–) were generated because of cyclic conversion between Fe2+ and Fe3+. Meanwhile, MoS2 nanoflowers with a high specific surface area could not only load more Fe3O4 but also solve its agglomeration problem, which greatly improved the catalytic efficiency. Moreover, a biosensor chip was constructed by standard lithography processes for disease detection, which had good sensitivity and portability. According to the above strategies, the developed portable sensing platform played the part of promoting the practical application of bioanalysis in early tumor screening and clinical diagnosis. In addition, we developed a short peptide ligand (NARKFYKG, NAR) to avoid the occupation of antigen binding sites by specifically connecting to Fc fragments in antibodies. Thus, the binding efficiency of antibodies and the activity of biosensors were improved due to the introduction of NAR.
中文翻译：
高效CdSe量子点/Fe3O4@MoS2/S2O82——基于微流控分析平台的电化学发光系统灵敏检测神经元特异性烯醇化酶
本工作基于电化学发光（ECL）技术和自组装便携式疾病检测芯片，开发了一种生物活性维持的传感平台，用于神经元特异性烯醇化酶的定量检测。首先，我们制备了 Fe 3 O 4 @MoS 2纳米复合材料作为加速过硫酸盐（S 2 O 8 2- ）还原的有效催化剂。具体来说，由于Fe 2+和Fe 3+之间的循环转化，产生了大量的硫酸根（SO 4 •– ） 。同时，具有高比表面积的MoS 2纳米花不仅可以负载更多的Fe 3 O4还解决了它的团聚问题，大大提高了催化效率。此外，通过标准光刻工艺构建了用于疾病检测的生物传感器芯片，具有良好的灵敏度和便携性。根据上述策略，开发的便携式传感平台为促进生物分析在早期肿瘤筛查和临床诊断中的实际应用发挥了作用。此外，我们开发了一种短肽配体（NARKFYKG，NAR），通过特异性连接抗体中的 Fc 片段来避免占用抗原结合位点。因此，由于引入了 NAR，抗体的结合效率和生物传感器的活性得到了提高。
更新日期：2022-06-16
中文翻译：
高效CdSe量子点/Fe3O4@MoS2/S2O82——基于微流控分析平台的电化学发光系统灵敏检测神经元特异性烯醇化酶
本工作基于电化学发光（ECL）技术和自组装便携式疾病检测芯片，开发了一种生物活性维持的传感平台，用于神经元特异性烯醇化酶的定量检测。首先，我们制备了 Fe 3 O 4 @MoS 2纳米复合材料作为加速过硫酸盐（S 2 O 8 2- ）还原的有效催化剂。具体来说，由于Fe 2+和Fe 3+之间的循环转化，产生了大量的硫酸根（SO 4 •– ） 。同时，具有高比表面积的MoS 2纳米花不仅可以负载更多的Fe 3 O4还解决了它的团聚问题，大大提高了催化效率。此外，通过标准光刻工艺构建了用于疾病检测的生物传感器芯片，具有良好的灵敏度和便携性。根据上述策略，开发的便携式传感平台为促进生物分析在早期肿瘤筛查和临床诊断中的实际应用发挥了作用。此外，我们开发了一种短肽配体（NARKFYKG，NAR），通过特异性连接抗体中的 Fc 片段来避免占用抗原结合位点。因此，由于引入了 NAR，抗体的结合效率和生物传感器的活性得到了提高。
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