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Sesamin Activates Skeletal Muscle FNDC5 Expression and Increases Irisin Secretion via the SIRT1 Signaling Pathway
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-16 , DOI: 10.1021/acs.jafc.2c02794 Guangning Kou 1, 2 , Peiyuan Li 1, 2 , Yanfei Shi 1 , Stanislav Seydou Traore 2 , Xiaoyang Shi 1 , Adwoa Nyantakyiwaa Amoah 2 , Zhenwei Cui 1 , Quanjun Lyu 3
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-16 , DOI: 10.1021/acs.jafc.2c02794 Guangning Kou 1, 2 , Peiyuan Li 1, 2 , Yanfei Shi 1 , Stanislav Seydou Traore 2 , Xiaoyang Shi 1 , Adwoa Nyantakyiwaa Amoah 2 , Zhenwei Cui 1 , Quanjun Lyu 3
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Sesamin, a major lignin mainly found in sesame (Sesamum indicum) oil and sesame seeds, has been demonstrated to possess lipoclasis-promoting, antiobesity, and antidiabetic effects. Irisin is a newly discovered myokine that has attracted great interest as a key target to prevent/treat obesity and its related metabolic diseases. However, the effect and potential mechanism of sesamin on FNDC5/irisin are still vacant. In this study, we showed that sesamin treatment increased FNDC5/irisin activation and regulated SIRT1, PGC-1α, and p-SMAD3/SMAD3 expression in C2C12 cells. By using specific inhibitors and lentivirus in C2C12 cells, we found that the SIRT1/SMAD3 axis plays an important role in sesamin regulated FNDC5/irisin activation. We also found that sesamin treatment activated FNDC5 expression and regulated the SIRT1/SMAD3 signaling axis in mice’s skeletal muscle. What is more, by the high-fat diet induced obese model, we further showed that sesamin improved the high-fat diet induced decrease in irisin production and secretion, which results in an improvement of body weight gain and skeletal muscle dysfunction. Our results suggested that sesamin could activate FNDC5 expression and stimulate irisin secretion through the SIRT1 pathway both in vitro and in vivo, which may provide a new strategy for preventing and improving irisin deficiency related diseases.
中文翻译：
芝麻素通过 SIRT1 信号通路激活骨骼肌 FNDC5 表达并增加鸢尾素分泌
芝麻素，一种主要存在于芝麻中的主要木质素（Sesamum indicum) 油和芝麻，已被证明具有促进脂肪分解、抗肥胖和抗糖尿病的作用。鸢尾素是一种新发现的肌动蛋白，作为预防/治疗肥胖及其相关代谢疾病的关键靶点引起了极大的兴趣。然而，芝麻素对FNDC5/irisin的作用和潜在机制尚不清楚。在这项研究中，我们发现芝麻素处理增加了 FNDC5/鸢尾素的活化并调节了 C2C12 细胞中 SIRT1、PGC-1α 和 p-SMAD3/SMAD3 的表达。通过在 C2C12 细胞中使用特异性抑制剂和慢病毒，我们发现 SIRT1/SMAD3 轴在芝麻素调节的 FNDC5/irisin 激活中起重要作用。我们还发现芝麻素处理激活了 FNDC5 的表达并调节了小鼠骨骼肌中的 SIRT1/SMAD3 信号轴。更，通过高脂饮食诱导的肥胖模型，我们进一步表明芝麻素改善了高脂饮食诱导的鸢尾素产生和分泌减少，从而改善了体重增加和骨骼肌功能障碍。我们的研究结果表明，芝麻素可以通过 SIRT1 通路激活 FNDC5 表达并刺激鸢尾素分泌。体外和体内研究，可能为预防和改善鸢尾素缺乏相关疾病提供新的策略。
更新日期：2022-06-16
中文翻译：
芝麻素通过 SIRT1 信号通路激活骨骼肌 FNDC5 表达并增加鸢尾素分泌
芝麻素，一种主要存在于芝麻中的主要木质素（Sesamum indicum) 油和芝麻，已被证明具有促进脂肪分解、抗肥胖和抗糖尿病的作用。鸢尾素是一种新发现的肌动蛋白，作为预防/治疗肥胖及其相关代谢疾病的关键靶点引起了极大的兴趣。然而，芝麻素对FNDC5/irisin的作用和潜在机制尚不清楚。在这项研究中，我们发现芝麻素处理增加了 FNDC5/鸢尾素的活化并调节了 C2C12 细胞中 SIRT1、PGC-1α 和 p-SMAD3/SMAD3 的表达。通过在 C2C12 细胞中使用特异性抑制剂和慢病毒，我们发现 SIRT1/SMAD3 轴在芝麻素调节的 FNDC5/irisin 激活中起重要作用。我们还发现芝麻素处理激活了 FNDC5 的表达并调节了小鼠骨骼肌中的 SIRT1/SMAD3 信号轴。更，通过高脂饮食诱导的肥胖模型，我们进一步表明芝麻素改善了高脂饮食诱导的鸢尾素产生和分泌减少，从而改善了体重增加和骨骼肌功能障碍。我们的研究结果表明，芝麻素可以通过 SIRT1 通路激活 FNDC5 表达并刺激鸢尾素分泌。体外和体内研究，可能为预防和改善鸢尾素缺乏相关疾病提供新的策略。
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