Herein, a multifunctional pentagon DNA nanostructure (MPDN) was assembled by the hybridization of a circular DNA scaffold containing five different fragments with five diverse DNA oligonucleotides for simultaneous sensitive detection and accurate colocalization imaging of dual-miRNAs in cancer cells. Exactly, the MPDN could specifically and efficiently internalize into folate (FA) receptor-overexpressed cells via specific binding of FA and the FA receptor to distinguish cancer cells from normal cells and transform trace amounts of targets miRNA-21 and miRNA-155 into substantial FAM and Cy5-labeled DNA polymers as the signal probe to generate two remarkable fluorescence emissions, realizing simultaneously sensitive detection of dual-miRNAs. Impressively, compared with traditional small fragment DNA probes with high fluidity, the DNA copolymers with extremely low diffusivity kept it in the originally generated position to achieve the colocalization imaging of dual-miRNAs more accurately for revealing the spatial expression information of dual-miRNAs in tissues and cells. This strategy provided programmable tool to simultaneously detect and accurately colocate dual-miRNAs for understanding normal physiology and the tumor mechanism.

中文翻译：

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基于多功能五边形 DNA 纳米结构的共轭链反应的癌细胞中双 miRNA 的超灵敏荧光检测和准确共定位可视化

在此，多功能五边形 DNA 纳米结构 (MPDN) 通过包含五个不同片段的圆形 DNA 支架与五种不同的 DNA 寡核苷酸的杂交组装而成，用于同时对癌细胞中的双 miRNA 进行灵敏检测和准确共定位成像。确切地说，MPDN 可以通过 FA 和 FA 受体的特异性结合，特异性有效地内化到叶酸 (FA) 受体过表达的细胞中，以区分癌细胞和正常细胞，并将微量的靶 miRNA-21 和 miRNA-155 转化为大量的 FAM Cy5标记的DNA聚合物作为信号探针产生两种显着的荧光发射，实现双miRNA的同时灵敏检测。令人印象深刻的是，与具有高流动性的传统小片段 DNA 探针相比，具有极低扩散率的DNA共聚物将其保持在最初生成的位置，从而更准确地实现dual-miRNAs的共定位成像，从而揭示dual-miRNAs在组织和细胞中的空间表达信息。该策略提供了可编程工具，可以同时检测和准确定位双 miRNA，以了解正常生理学和肿瘤机制。