Although aging is the biggest risk factor for human chronic (cancer, diabetic, cardiovascular, and neurodegenerative) diseases, few interventions are known besides caloric restriction and a small number of drugs (with substantial side effects) that directly address aging. Thus, there is an urgent need for new options that can generally delay aging processes and prevent age-related diseases. Cellular aging is at the basis of aging processes. Chronological lifespan (CLS) of yeast Saccharomyces cerevisiae is the well-established model system for investigating the interventions of human post-mitotic cellular aging. CLS is defined as the number of days cells remain viable in a stationary phase. We developed a new, cheap, and fast quantitative method for measuring CLS in cell cultures incubated together with various chemical agents and controls on 96-well plates. Our PICLS protocol with (1) the use of propidium iodide for fluorescent-based cell survival reading in a microplate reader and (2) total cell count measurement via OD600nm absorption from the same plate provides real high-throughput capacity. Depending on logistics, large numbers of plates can be processed in parallel so that the screening of thousands of compounds becomes feasible in a short time. The method was validated by measuring the effect of rapamycin and calorie restriction on yeast CLS. We utilized this approach for chemical agent screening. We discovered the anti-aging/geroprotective potential of 2,5-anhydro-D-mannitol (2,5-AM) and suggest its usage individually or in combination with other anti-aging interventions.

尽管衰老是人类慢性（癌症、糖尿病、心血管和神经退行性）疾病的最大风险因素，但除了热量限制和少量直接解决衰老问题的药物（具有显着副作用）外，已知的干预措施很少。因此，迫切需要新的选择，通常可以延缓衰老过程并预防与年龄有关的疾病。细胞老化是老化过程的基础。酿酒酵母的时间寿命 (CLS)是用于研究人类有丝分裂后细胞衰老干预的成熟模型系统。CLS 定义为细胞在静止期保持活力的天数。我们开发了一种新的、廉价且快速的定量方法，用于测量在 96 孔板上与各种化学试剂和对照一起孵育的细胞培养物中的 CLS。我们的 PICLS 协议包括 (1) 使用碘化丙啶在微孔板读取器中进行基于荧光的细胞存活读数，以及 (2) 通过同一板的 OD600nm 吸收测量总细胞计数，提供真正的高通量能力。根据物流，可以并行处理大量板，以便在短时间内筛选数千种化合物变得可行。通过测量雷帕霉素和热量限制对酵母 CLS 的影响来验证该方法。我们利用这种方法进行化学试剂筛选。我们发现了 2,5-脱水-D-甘露醇 (2,5-AM) 的抗衰老/抗衰老潜力，并建议单独使用或与其他抗衰老干预措施联合使用。