The opportunistic pathogen Pseudomonas aeruginosa has evolved several systems to adapt to complex environments. The GntR family proteins play important roles in the regulation of metabolic processes and bacterial pathogenesis. In this study, we uncovered that the gene clusters of PA1513-PA1518 and PA1498-PA1502 in P. aeruginosa are required for uric acid and glyoxylate metabolism respectively. We also identified a GntR family regulator UgmR that is involved in regulation of uric acid and glyoxylate metabolism. Promoter activity measurement and biochemical assays revealed that the UgmR directly represses the transcriptional activity of PA1513-PA1518 and PA1498-PA1502, and this inhibition was relieved by the addition of uric acid. Importantly, further experiments showed that UgmR also participates in the glyoxylate shunt. Collectively, these findings contribute to a better understanding of the UgmR factor involved in uric acid and glyoxylate metabolism, which provide insights into the complex metabolic pathways in P. aeruginosa.

中文翻译：

---

UgmR蛋白对铜绿假单胞菌尿酸和乙醛酸代谢的调控

机会性病原体铜绿假单胞菌已经进化出几个系统来适应复杂的环境。GntR 家族蛋白在代谢过程和细菌发病机制的调节中起重要作用。在这项研究中，我们发现了P中 PA1513-PA1518 和 PA1498-PA1502 的基因簇。绿脓杆菌分别是尿酸和乙醛酸代谢所必需的。我们还鉴定了一个参与调节尿酸和乙醛酸代谢的 GntR 家族调节因子 UgmR。启动子活性测量和生化测定表明，UgmR 直接抑制 PA1513-PA1518 和 PA1498-PA1502 的转录活性，并且这种抑制通过添加尿酸得到缓解。重要的是，进一步的实验表明 UgmR 也参与乙醛酸分流。总的来说，这些发现有助于更好地理解参与尿酸和乙醛酸代谢的 UgmR 因子，这为了解P中复杂的代谢途径提供了见解。绿脓杆菌。