Background

Many guidelines and safety measures led to a decrease in exposure to antineoplastic agents. Since healthcare workers are often exposed to lower concentrations than patients, a sensitive method is needed to quantify occupational exposure.

Objective

The aim of this study was to develop and validate a sensitive method for simultaneous detection and quantification of cyclophosphamide, ifosfamide and paclitaxel in urine by use of UPLC-MS/MS with a UniSpray ionisation source.

Methods

Compounds were extracted from urine using Novum simplified liquid extraction cartridges, separated on a C18 column, ionised by a UniSpray ionisation source and detected with MS/MS. In the second part of the study, a field study was performed to assess occupational exposure to antineoplastic agents.

Results

Eighty-three samples from healthcare workers were analysed and resulted in seventeen samples containing quantifiable concentrations of at least one compound. In conclusion, a sensitive method for simultaneous detection and quantification of cyclophosphamide (LLOQ 0.05 ng/mL), ifosfamide (LLOQ 0.3 ng/mL) and paclitaxel (LLOQ 0.7 ng/mL) was developed and validated.

中文翻译：

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使用 UniSpray 电离源对尿液中的三种抗肿瘤药物进行定量

背景

许多指南和安全措施导致抗肿瘤药物的暴露减少。由于医护人员的暴露浓度通常低于患者，因此需要一种敏感的方法来量化职业暴露。

客观的

本研究的目的是开发和验证一种灵敏的方法，通过使用带有 UniSpray 电离源的 UPLC-MS/MS 同时检测和定量尿液中的环磷酰胺、异环磷酰胺和紫杉醇。

方法

使用 Novum 简化液体萃取柱从尿液中提取化合物，在 C18 柱上分离，由 UniSpray 电离源电离，并用 MS/MS 检测。在研究的第二部分，进行了一项实地研究，以评估抗肿瘤药物的职业暴露。

结果

对来自医护人员的 83 个样本进行了分析，得到的 17 个样本含有至少一种可量化浓度的化合物。总之，开发并验证了一种同时检测和定量环磷酰胺（LLOQ 0.05 ng/mL）、异环磷酰胺（LLOQ 0.3 ng/mL）和紫杉醇（LLOQ 0.7 ng/mL）的灵敏方法。