cis,cis-Muconate (ccMA) is a promising platform for use in synthesizing various polymers. A glucose-free ccMA production using Pseudomonas sp. NGC7 from hardwood lignin-derived aromatic compounds was previously reported. In that system, syringyl nucleus compounds were essential for growth. Here, it is shown that NGC7 is available for glucose-free ccMA production even from a mixture of lignin-derived aromatics that does not contain syringyl nucleus compounds. By introducing a gene set for the protocatechuate (PCA)-shunt consisting of PCA 3,4-dioxygenase and PCA decarboxylase into an NGC7-derived strain deficient in PCA 3,4-dioxygenase and ccMA cycloisomerase, it was succeeded in constructing a ccMA-producing strain that grows on a lignin-derived aromatics mixture containing no syringyl nucleus compounds. Finally, it is demonstrated that the engineered strain produced ccMA from sugar cane bagasse alkaline extract in 18.7 mol%. NGC7 is thus shown to be a promising microbial chassis for biochemicals production from lignin-derived aromatics.

cis,cis -Muconate (ccMA) 是用于合成各种聚合物的有前途的平台。使用假单胞菌的无葡萄糖 ccMA 生产sp。先前报道了来自硬木木质素衍生的芳香族化合物的 NGC7。在该系统中，紫丁香环化合物对生长至关重要。在这里，表明 NGC7 可用于不含葡萄糖的 ccMA 生产，即使是由不含紫丁香基核化合物的木质素衍生芳烃混合物制成。通过将由 PCA 3,4-双加氧酶和 PCA 脱羧酶组成的原儿茶酸 (PCA)-分流基因组引入缺乏 PCA 3,4-双加氧酶和 ccMA 环异构酶的 NGC7 衍生菌株，成功构建了 ccMA-产生在不含紫丁香基核化合物的木质素衍生芳烃混合物上生长的菌株。最后，证明工程菌株从甘蔗渣碱性提取物中产生 18.7 mol% 的 ccMA。