Use of spin labels to study structures of polymers has been widely spread in polymer science. However, for the studies of neutral water-soluble dietary fibers (DFs), labelling efficiencies in past studies have only been sufficient for application of continuous wave electron paramagnetic resonance spectroscopy (CW-EPR), but still insufficient for some advanced methods such as pulse EPR. Thus, in this paper, two spin labelling strategies, namely, site-selective mono-spin-labelling and stochastic multi-spin-labelling, were examined on linear cereal β-glucan, as well as linearly branched arabinoxylan and galactomannan. The effects of both methods in DF properties were evaluated. For the mono-labelling pathway, labelling efficiency could reach up to 46 %. In the stochastic labelling strategy, a degree of substitution (DS) up to 150 % could be reached, whereas optimized conditions for this strategy were achieved at DS = 3 % to obtain DFs whose bioactivity properties were still preserved while spin labelling efficiency was still sufficient for CW and pulse EPR experiments.

使用自旋标签研究聚合物的结构已在聚合物科学中广泛传播。然而，对于中性水溶性膳食纤维（DFs）的研究，过去研究的标记效率仅足以应用连续波电子顺磁共振波谱（CW-EPR），但对于脉冲等一些先进方法仍然不够EPR。因此，在本文中，在线性谷物β-葡聚糖以及线性支化的阿拉伯木聚糖和半乳甘露聚糖上检测了两种自旋标记策略，即位点选择性单自旋标记和随机多自旋标记。评估了两种方法在 DF 属性中的效果。对于单标记途径，标记效率可达 46%。在随机标记策略中，可以达到高达 150% 的替代度 (DS)，